phospho-EGFR (Tyr-1101) Peptide | c-erbB proto-oncogene peptide
phospho-EGFR (Tyr-1101) Peptide
Synonyms
phospho-EGFR (Tyr-1101) Peptide; c-erbB proto-oncogene peptide
Specificity
The peptide is specifically recognized by EGFR (Tyr-1101) antibody (EM1991) in ELISA, and has been shown to block the reactivity of EM1991 in Western blot and immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: Phospho-EGFR (Tyr-1101) synthetic peptide corresponding to amino acid residues surrounding tyrosine 1101 of human EGFR (ErbB-1). This human EGFR sequence has high homology to rat and mouse EGFR, and is not conserved in other ErbB family members.
Sequence Length
10
Application Notes
ELISA: 50 ng/well
Immunocytochemistry: 1:500
Western Blot: 1:1,000
Immunocytochemistry: 1:500
Western Blot: 1:1,000
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Related Product Information for c-erbB proto-oncogene peptide
The epidermal growth factor receptor (EGFR) is a transmembrane glycoprotein with an extracellular ligand-binding domain and a cytoplasmic domain with intrinsic tyrosine kinase activity. The cytoplasmic domain has a C-terminal region with multiple autophosphorylation sites (Tyr-992, 1068, 1086, 1148, and 1173). These sites are important for downstream signaling and rapid internalization. In addition, EGFR activation leads to c-Src mediated phosphorylation of Tyr-845 and Tyr-1101. The former site is required for mitogenic responses to EGFR activation, while the latter may be an SH2 binding site. Phosphorylation of EGFR on serine and threonine residues is thought to represent a mechanism for regulation of receptor kinase activity and internalization. These sites include a PKC site (Thr-654), CAMKII sites (Ser-1046, 1047, 1057, and 1142), and constitutively phosphorylated sites (Ser-967 and Ser-1002). Thus, the regulation of EGFR activity involves a complex series of phosphorylation events at multiple sites throughout the intracellular portion of the receptor.
References
• Carpenter, G. (2000) Bioessays 22:697.
• Boeri Erba, E. et al. (2005) Mol. Cell. Prot. 4:1107.
• Boeri Erba, E. et al. (2005) Mol. Cell. Prot. 4:1107.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
1,020 Da
NCBI Official Full Name
epidermal growth factor receptor
NCBI Official Symbol
c-erbB proto-oncogene
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
c-erbB proto-oncogene
UniProt Entry Name
Q9P2Z9_HUMAN
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Product Notes
The c-erbB proto-oncogene c-erbb proto-oncogene (Catalog #AAA474035) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. ELISA: 50 ng/well Immunocytochemistry: 1:500 Western Blot: 1:1,000. Researchers should empirically determine the suitability of the c-erbB proto-oncogene c-erbb proto-oncogene for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Phospho-EG FR (Tyr-1101) synthetic peptide correspond ing to amino acid residues surroundin g tyrosine 1101 of human EGFR (ErbB-1). This human EGFR sequence has high homology to rat and mouse EGFR, and is not conserved in other ErbB family members. It is sometimes possible for the material contained within the vial of "phospho-EGFR (Tyr-1101) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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