Testing Data
C-Raf (C-terminus) Peptide | raf1a peptide
C-Raf (C-terminus) Peptide
Gene Names
raf1a; craf; raf1; c-raf
Synonyms
C-Raf (C-terminus) Peptide; raf1a peptide
Specificity
The peptide is specifically recognized by anti-C-Raf (Cterminus) antibody (RP2071) in ELISA, and has been shown to block the reactivity of RP2071 during Western blot. In addition, the peptide is recommended for use in blocking RP2071 reactivity in immunocytochemistry.
Form/Format
Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.
Sequence
Peptide Sequence: C-Raf (C-terminus) synthetic peptide corresponding to amino acids 637 to 648 in human C-Raf. This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf.
Application Notes
Blocking: 1:1,000
ELISA: 50 ng/well
ELISA: 50 ng/well
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Testing Data
Testing Data
Related Product Information for raf1a peptide
The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, BRaf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (S259)/B-Raf (S365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. EGF receptor activation leads to phosphorylation of Ser-471, which is critical for C-Raf kinase activity and is required for interaction with MEK. In B-Raf, the corresponding conserved site is Ser-578, and mutation of this residue to alanine produces an inactivate kinase. Thus, this Raf phosphorylation site may be critical for kinase activity and may be important for MEK binding and activation.
References
• Mason, C.S. et al. (1999) EMBOJ 18(8):2137.
• Zhu, J. et al. (2005). Mol. Biol. Cell 16:4733.
• Zhu, J. et al. (2005). Mol. Biol. Cell 16:4733.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,871 Da
NCBI Official Full Name
c-raf
NCBI Official Synonym Full Names
v-raf-1 murine leukemia viral oncogene homolog 1a
NCBI Official Symbol
raf1a
NCBI Official Synonym Symbols
craf; raf1; c-raf
NCBI Protein Information
RAF proto-oncogene serine/threonine-protein kinase; serine/threonine protein kinase RAF1; murine leukemia viral oncogene homolog; v-raf-1 murine leukemia viral oncogene homolog 1
UniProt Protein Name
C-raf protein
UniProt Gene Name
raf1a
UniProt Synonym Gene Names
c-raf
UniProt Entry Name
Q90458_DANRE
Research Articles on raf1a
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Product Notes
The raf1a raf1a (Catalog #AAA474008) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Blocking: 1:1,000 ELISA: 50 ng/well. Researchers should empirically determine the suitability of the raf1a raf1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: C-Raf (C-terminu s) synthetic peptide correspond ing to amino acids 637 to 648 in human C-Raf. This sequence is conserved in C-Rafs from many species, and is not found in A-Raf or B-Raf. It is sometimes possible for the material contained within the vial of "C-Raf (C-terminus) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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