PDZK1 recombinant protein

PDZK1, 1-519aa, Human His tag, E Coli

Cat. Num. AAA205282

• Gene Names: PDZK1; CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PDZK1; 1-519aa; Human His tag; E Coli; Na (+)/H (+) exchange regulatory cofactor NHE-RF3; CAP70; CLAMP; NHERF-3; NHERF3; PDZD1; PDZK1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT.
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTSTFNP RECKLSKQEG QNYGFFLRIE KDTEGHLVRV VEKCSPAEKA GLQDGDRVLR INGVFVDKEE HMQVVDLVRK SGNSVTLLVL DGDSYEKAVK TRVDLKELGQ SQKEQGLSDN ILSPVMNGGV QTWTQPRLCY LVKEGGSYGF SLKTVQGKKG VYMTDITPQG VAMRAGVLAD DHLIEVNGEN VEDASHEEVV EKVKKSGSRV MFLLVDKETD KRHVEQKIQF KRETASLKLL PHQPRIVEMK KGSNGYGFYL RAGSEQKGQI IKDIDSGSPA EEAGLKNNDL VVAVNGESVE TLDHDSVVEM IRKGGDQTSL LVVDKETDNM YRLAHFSPFL YYQSQELPNG SVKEAPAPTP TSLEVSSPPD TTEEVDHKPK LCRLAKGENG YGFHLNAIRG LPGSFIKEVQ KGGPADLAGL EDEDVIIEVN GVNVLDEPYE KVVDRIQSSG KNVTLLVCGK KAYDYFQAKK IPIVSSLADP LDTPPDSKEG IVVESNHDSH MAKERAHSTA SHSSSNSEDT EM
• Sequence Length: 519

• Applicable Applications for PDZK1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PDZK1 recombinant protein

PDZK1 is a PDZ domain-containing scaffolding protein. This protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Recombinant human PDZK1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PDZK1 recombinant protein

Metabolism

References

Kocher O, Yesilaltay A, et al. (2003). J Biol Chem. 278 (52):52820-5.; Inoue J, Otsuki T, et al. (2004). Am J Pathol. 165 (1):71-81.;

NCBI and Uniprot Product Information

• NCBI GI #: 318037203
• NCBI GeneID: 5174
• NCBI Accession #: NP_001188254
• NCBI GenBank Nucleotide #: NM_001201325.1
• UniProt Accession #: Q5T2W1; O60450; Q5T5P6; Q9BQ41; B4DPB9; E7EU02
• Molecular Weight: 59 kDa (542aa)
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3 isoform 1
• NCBI Official Synonym Full Names: PDZ domain containing 1
• NCBI Official Symbol: PDZK1
• NCBI Official Synonym Symbols: CAP70; CLAMP; PDZD1; NHERF3; NHERF-3
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• Protein Family: PDZK1-interacting protein
• UniProt Gene Name: PDZK1
• UniProt Synonym Gene Names: CAP70; NHERF3; PDZD1; NHERF-3; NaPi-Cap1

NCBI Description

This gene encodes a PDZ domain-containing scaffolding protein. PDZ domain-containing molecules bind to and mediate the subcellular localization of target proteins. The encoded protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Pseudogenes of this gene are located on the long arm of chromosome 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. Required for normal cell-surface expression of SCARB1. Plays a role in maintaining normal plasma cholesterol levels via its effects on SCARB1. Plays a role in the normal localization and function of the chloride-anion exchanger SLC26A6 to the plasma membrane in the brush border of the proximal tubule of the kidney. May be involved in the regulation of proximal tubular Na+-dependent inorganic phosphate cotransport therefore playing an important role in tubule function ().