Human protein disulfide isomerase family A, member 3 ELISA Kit | PDIA3 elisa kit

Human protein disulfide-isomerase A3, PDIA3 ELISA Kit

Cat. Num. AAA700585

• Gene Names: PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• Reactivity: Human
• Synonyms: protein disulfide isomerase family A; member 3; Human protein disulfide-isomerase A3; PDIA3 ELISA Kit; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; HsT17083; P58; PI-PLC; 58 kDa microsomal protein; endoplasmic reticulum P58; glucose regulated protein; 58kDa; phospholipase C-alpha; protein disulfide isomerase; PDIA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human PDIA3. No significant cross-reactivity or interference between human PDIA3 and analogues was observed.
• Sequence Length: 505

• Samples: Serum, plasma, cell culture supernates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.25 ng/ml-80 ng/ml
• Sensitivity: < 0.31 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDIA3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDIA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDIA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDIA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDIA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21361657
• NCBI GeneID: 2923
• NCBI Accession #: NP_005304.3
• NCBI GenBank Nucleotide #: NM_005313.4
• UniProt Accession #: P30101; Q13453; Q14255; Q8IYF8; Q9UMU7; AAB37397
• Molecular Weight: 56,782 Da
• NCBI Official Full Name: protein disulfide-isomerase A3
• NCBI Official Synonym Full Names: protein disulfide isomerase family A, member 3
• NCBI Official Symbol: PDIA3
• NCBI Official Synonym Symbols: P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• NCBI Protein Information: protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
• UniProt Protein Name: Protein disulfide-isomerase A3
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA3
• UniProt Synonym Gene Names: ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
• UniProt Entry Name: PDIA3_HUMAN

NCBI Description

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: EC 5.3.4.1; Endoplasmic reticulum; Isomerase; Protease; Apoptosis; Chaperone; Phospholipase

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: focal adhesion; cell surface; smooth endoplasmic reticulum; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus

Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; protein import into nucleus; cell redox homeostasis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I; protein amino acid N-linked glycosylation via asparagine; signal transduction; proteolysis; post-translational protein modification; protein retention in ER