Human Protein Disulfide Isomerase A3 ELISA Kit | PDIA3 elisa kit

Human Protein Disulfide Isomerase A3 ELISA Kit

Cat. Num. AAA731408

• Gene Names: PDIA3; P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• Reactivity: Human
• Synonyms: Protein Disulfide Isomerase A3; Human Protein Disulfide Isomerase A3 ELISA Kit; PDIA3 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PDIA3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDIA3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDIA3 elisa kit

Principle of the Assay: PDIA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDIA3 antibody and an PDIA3-HRP conjugate. The assay sample and buffer are incubated together with PDIA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDIA3 concentration since PDIA3 from samples and PDIA3-HRP conjugate compete for the anti-PDIA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDIA3 from the sample, fewer sites are left to bind PDIA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDIA3 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21361657
• NCBI GeneID: 2923
• NCBI Accession #: NP_005304.3
• NCBI GenBank Nucleotide #: NM_005313.4
• Molecular Weight: 56,782 Da
• NCBI Official Full Name: protein disulfide-isomerase A3
• NCBI Official Synonym Full Names: protein disulfide isomerase family A, member 3
• NCBI Official Symbol: PDIA3
• NCBI Official Synonym Symbols: P58; ER60; ERp57; ERp60; ERp61; GRP57; GRP58; PI-PLC; HsT17083; HEL-S-269; HEL-S-93n
• NCBI Protein Information: protein disulfide-isomerase A3; ER protein 57; ER protein 60; phospholipase C-alpha; 58 kDa microsomal protein; disulfide isomerase ER-60; endoplasmic reticulum P58; 58 kDa glucose-regulated protein; glucose regulated protein, 58kDa; epididymis secretory protein Li 269; protein disulfide isomerase-associated 3; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 93n
• UniProt Protein Name: Protein disulfide-isomerase A3
• Protein Family: Protein disulfide-isomerase
• UniProt Gene Name: PDIA3
• UniProt Synonym Gene Names: ERP57; ERP60; GRP58; p58; ER protein 57; ERp57; ER protein 60; ERp60
• UniProt Entry Name: PDIA3_HUMAN

NCBI Description

This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]

Uniprot Description

GRP58: a protein disulfide isomerase found in the endoplasmic reticulum lumen. Catalyzes the rearrangement of both intrachain and interchain disulfide bonds in proteins to form the native structures. An essential component of the peptide-loading complex of the major histocompatibility complex class I pathway.

Protein type: Protease; Isomerase; Apoptosis; Chaperone; EC 5.3.4.1; Phospholipase; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: smooth endoplasmic reticulum; focal adhesion; cell surface; endoplasmic reticulum lumen; endoplasmic reticulum; melanosome; nucleus

Molecular Function: disulfide oxidoreductase activity; protein binding; cysteine-type endopeptidase activity; protein disulfide isomerase activity; phospholipase C activity

Biological Process: antigen processing and presentation of peptide antigen via MHC class I; cellular protein metabolic process; protein folding; cell redox homeostasis; protein import into nucleus; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; protein amino acid N-linked glycosylation via asparagine; proteolysis; post-translational protein modification; signal transduction; protein retention in ER