Human Platelet-derived growth factor D ELISA Kit | PDGFD elisa kit
Human Platelet-derived growth factor D (PDGFD) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PDGF-BB in Human serum, plasma. An antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-BB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-BB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11q22.3
Cellular Component: endoplasmic reticulum lumen; extracellular exosome; extracellular region; extracellular space; Golgi membrane
Molecular Function: growth factor activity; platelet-derived growth factor receptor binding
Biological Process: blood coagulation; cellular response to hydrogen peroxide; cellular response to transforming growth factor beta stimulus; multicellular organism development; platelet-derived growth factor receptor signaling pathway; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of fibroblast proliferation; positive regulation of glomerular mesangial cell proliferation; positive regulation of MAP kinase activity; positive regulation of monocyte extravasation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of smooth muscle cell chemotaxis; positive regulation of smooth muscle cell proliferation; regulation of peptidyl-tyrosine phosphorylation; regulation of receptor activity
Research Articles on PDGFD
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Product Notes
The Human PDGFD pdgfd (Catalog #AAA280444) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280444 ELISA Kit recognizes Human PDGFD. It is sometimes possible for the material contained within the vial of "Platelet-derived growth factor D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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