Chicken Platelet Derived Growth Factor D ELISA Kit | PDGFD elisa kit

Chicken Platelet Derived Growth Factor D ELISA Kit

Cat. Num. AAA741681

• Gene Names: PDGFD; IEGF; SCDGFB; MSTP036; SCDGF-B
• Reactivity: Chicken
• Synonyms: Platelet Derived Growth Factor D; Chicken Platelet Derived Growth Factor D ELISA Kit; PDGFD elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This PDGFD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken PDGFD. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDGFD elisa kit

Principle of the Assay: PDGFD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDGFD antibody and an PDGFD-HRP conjugate. The assay sample and buffer are incubated together with PDGFD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDGFD concentration since PDGFD from samples and PDGFD-HRP conjugate compete for the anti-PDGFD antibody binding site. Since the number of sites is limited, as more sites are occupied by PDGFD from the sample, fewer sites are left to bind PDGFD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDGFD concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PDGFD elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 15451921
• NCBI GeneID: 80310
• NCBI Accession #: NP_149126.1
• NCBI GenBank Nucleotide #: NM_033135.3
• UniProt Accession #: Q9GZP0; Q9BWV5; A8K9T6
• Molecular Weight: 42,848 Da
• NCBI Official Full Name: platelet-derived growth factor D isoform 2
• NCBI Official Synonym Full Names: platelet derived growth factor D
• NCBI Official Symbol: PDGFD
• NCBI Official Synonym Symbols: IEGF; SCDGFB; MSTP036; SCDGF-B
• NCBI Protein Information: platelet-derived growth factor D; PDGF-D; iris-expressed growth factor; spinal cord derived growth factor B; spinal cord-derived growth factor B; spinal cord-derived growth factor-B
• UniProt Protein Name: Platelet-derived growth factor D
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFD
• UniProt Synonym Gene Names: IEGF; SCDGFB; PDGF-D; SCDGF-B; PDGFD latent form
• UniProt Entry Name: PDGFD_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PDGFD: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Oncoprotein

Chromosomal Location of Human Ortholog: 11q22.3

Cellular Component: Golgi membrane; endoplasmic reticulum lumen; extracellular region

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding

Biological Process: positive regulation of cell division; multicellular organismal development; platelet-derived growth factor receptor signaling pathway; regulation of peptidyl-tyrosine phosphorylation