Platelet Derived Growth Factor-BB Recombinant Protein | PDGFBB recombinant protein

Human Platelet Derived Growth Factor-BB Recombinant

Cat. Num. AAA553042

• Purity: >98%, as determined by SDS-PAGE and HPLC.
• Synonyms: Platelet Derived Growth Factor-BB; Human Platelet Derived Growth Factor-BB Recombinant; Human PDGFBB Recombinant; pdgf-bb; platelet; PDGFBB recombinant protein

• Host: Saccharomyces Cerevisiae
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC.
• Form/Format: Recombinant Platelet Dreived Growth Factor-BB was lyophilized from a 0.2 um filtered 0.02M acetic acid solution pH 6.5.
• Sequence: MNRCWALFLS LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHG DPGEEDGAEL DLNMTRSHSG GELESLARGR RSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT RSPGGSQEQR AKTPQTRVTI RTVRVRRPPK GKHRKFKHTH DKTALKETLG A
• Sequence Length: 241

• Domain: cystine knot growth factor
• Host Note: Optimized DNA sequence encoding Human PDGF-BB mature chain was expressed in Saccharomyces cerevisiae.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human PDGF-BB, generated by the proteolytic removal of the signal peptide; and propeptide, the molecule has a calculated molecular mass of approximately 16 kDa. ; ; Recombinant PDGF-BB is a disulfide-linked homodimeric protein consisting of two 109 amino acid residue subunits. Recombinant PDGF-BB migrates due to glycosylation as an approximately 32 kDa protein under non-reducing conditions and as 16kDa under reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for PDGFBB recombinant protein

PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB,AB,AA) while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells.Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta (CD140b).In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor.

Product Categories/Family for PDGFBB recombinant protein

Cytokines

References

• paradoxical effects of PDGF-BB overexpression in endothelial cells on engineered blood vessels in vivo .Am. J. Pathol., Jul 2009; 175: 294 - 302.
• sp1-dependent activation of klf4 is required for PDGF-BB-induced phenotypic modulation of smooth muscle .Am J Physiol Heart Circ Physiol, Apr 2009; 296: H1027 - H1037.
• mycophenolic acid inhibits the autocrine pdgf-b synthesis and PDGF-BB-induced mrna expression of egr-1 in rat mesangial cells .Nephrol. Dial. Transplant., Jan 2009; 24: 52 - 61.
• recombinant PDGF-BB enhances platelet recovery in mice with radiation induced thromobocytopenia. .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1232.
• platelet-derived growth factor (pdgf) -bb mediates survival of leukemic large granular lymphocyte via an autocrine regulatory pathway .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 3803.