Human Platelet-derived growth factor subunit A ELISA Kit | PDGFA elisa kit

Human Platelet-derived growth factor subunit A ELISA Kit

Cat. Num. AAA764213

• Gene Names: PDGFA; PDGF1; PDGF-A
• Reactivity: Human
• Synonyms: Platelet-derived growth factor subunit A; Human Platelet-derived growth factor subunit A ELISA Kit; PDGFA (Platelet Derived Growth Factor Subunit A )/PDGF-A; PDGFA elisa kit

• Reactivity: Human
• Sequence Length: 211

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PDGFA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PDGFA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PDGFA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PDGFA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PDGFA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 77695917
• NCBI GeneID: 5154
• NCBI Accession #: NP_002598.4
• NCBI GenBank Nucleotide #: NM_002607.5
• Molecular Weight: 22,253 Da
• NCBI Official Full Name: platelet-derived growth factor subunit A isoform 1 preproprotein
• NCBI Official Synonym Full Names: platelet derived growth factor subunit A
• NCBI Official Symbol: PDGFA
• NCBI Official Synonym Symbols: PDGF1; PDGF-A
• NCBI Protein Information: platelet-derived growth factor subunit A
• UniProt Protein Name: Platelet-derived growth factor subunit A
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFA
• UniProt Synonym Gene Names: PDGF1; PDGF subunit A
• UniProt Entry Name: PDGFA_HUMAN

NCBI Description

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PDGFA: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cytokine; Cell cycle regulation; Secreted

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cell surface; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi membrane; microvillus

Molecular Function: collagen binding; growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: actin cytoskeleton organization and biogenesis; activation of MAPKK activity; alveolus development; angiogenesis; axon guidance; blood coagulation; cell activation; cell projection biogenesis; cell-cell signaling; epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; fibroblast growth factor receptor signaling pathway; gut development; hair follicle development; innate immune response; inner ear development; insulin receptor signaling pathway; MAPKKK cascade; negative chemotaxis; negative regulation of phosphatidylinositol biosynthetic process; nerve growth factor receptor signaling pathway; organ morphogenesis; phosphoinositide-mediated signaling; platelet activation; platelet degranulation; platelet-derived growth factor receptor signaling pathway; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; positive regulation of protein kinase B signaling cascade; Ras protein signal transduction; regulation of actin cytoskeleton organization and biogenesis; regulation of peptidyl-tyrosine phosphorylation; regulation of smooth muscle cell migration; response to drug; response to estradiol stimulus; response to hypoxia; response to inorganic substance; response to retinoic acid; response to wounding; skin development; small GTPase mediated signal transduction; transforming growth factor beta receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; wound healing