Cyclic di-GMP phosphodiesterase Gmr (gmr) Recombinant Protein | gmr recombinant protein

Recombinant Escherichia coli Cyclic di-GMP phosphodiesterase Gmr (gmr)

Cat. Num. AAA1079409

• Gene Names: gmr; ECK1280; JW1278; yciR
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Cyclic di-GMP phosphodiesterase Gmr (gmr); Recombinant Escherichia coli Cyclic di-GMP phosphodiesterase Gmr (gmr); gmr recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-661, full length protein
• Sequence: MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTLQLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAVARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLFMSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLICSGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDNFKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMASRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQFCVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPERGLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQTIFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGYSSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKEDAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA
• Sequence Length: 661

• Species: Escherichia coli (strain K12)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 16129246
• NCBI GeneID: 945868
• NCBI Accession #: NP_415801.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P77334
• Molecular Weight: 74,665 Da
• NCBI Official Full Name: cyclic-di-GMP phosphodiesterase; csgD regulator; modulator of RNase II stability
• NCBI Official Symbol: gmr
• NCBI Official Synonym Symbols: ECK1280; JW1278; yciR
• NCBI Protein Information: cyclic-di-GMP phosphodiesterase; csgD regulator; modulator of RNase II stability
• UniProt Protein Name: Cyclic di-GMP phosphodiesterase PdeR
• Protein Family: Cyclic di-GMP phosphodiesterase
• UniProt Gene Name: pdeR

NCBI Description

Gmr has an N-terminal PAS domain, a putative diguanylate cyclase GGDEF domain, then a C-terminal cyclic-di-GMP phosphodiesterase EAL domain. [More information is available at EcoGene: EG13415]. Gmr/YciR controls curli biosynthesis by reducing transcription of the regulator CsgD, probably by degradation of the signalling molecule c-di-GMP. [More information is available at EcoCyc: G6639].

Uniprot Description

Part of a signaling cascade that regulates curli biosynthesis. The cascade is composed of two cyclic-di-GMP (c-di-GMP) control modules, in which c-di-GMP controlled by the DgcE/PdeH pair (module I) regulates the activity of the DgcM/PdeR pair (module II), which in turn regulates activity of the transcription factor MlrA and expression of the master biofilm regulator csgD (PubMed:23708798). PdeR acts as a trigger enzyme that connects modules I and II. It inhibits DgcM and MlrA by direct interaction. Inhibition is relieved when PdeR binds and degrades c-di-GMP generated by module I (PubMed:17010156, PubMed:23708798).