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Cyclic di-GMP phosphodiesterase YfgF (yfgF) Recombinant Protein | yfgF recombinant protein

Recombinant Escherichia coli Cyclic di-GMP phosphodiesterase YfgF (yfgF), partial

Gene Names
yfgF; ECK2499; JW2488
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Cyclic di-GMP phosphodiesterase YfgF (yfgF); Recombinant Escherichia coli Cyclic di-GMP phosphodiesterase YfgF (yfgF); partial; yfgF recombinant protein
Ordering
For Research Use Only!
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-747
Sequence
MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMLVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA
Sequence Length
747
Species
Escherichia coli (strain K12)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
85,608 Da
NCBI Official Full Name
cyclic-di-GMP phosphodiesterase, anaerobic
NCBI Official Symbol
yfgF
NCBI Official Synonym Symbols
ECK2499; JW2488
NCBI Protein Information
cyclic-di-GMP phosphodiesterase, anaerobic
UniProt Protein Name
Cyclic di-GMP phosphodiesterase PdeF
UniProt Gene Name
pdeF

NCBI Description

YfgF has a central GGDEF putative diguanylate cyclase, then a C-terminal putative cyclic-di-GMP phosphodiesterase EAL domain. [More information is available at EcoGene: EG14202]. YfgF is an inner membrane protein with nine predicted transmembrane domains. [More information is available at EcoCyc: G7314].

Uniprot Description

Phosphodiesterase (PDE) that catalyzes the hydrolysis of cyclic-di-GMP (c-di-GMP) to 5'-pGpG. Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di-GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.

Research Articles on yfgF

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Product Notes

The yfgF pdef (Catalog #AAA1085235) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-747. The amino acid sequence is listed below: MKLNATYIKI RDKWWGLPLF LPSLILPIFA HINTFAHISS GEVFLFYLPL ALMISMMMFF SWAALPGIAL GIFVRKYAEL GFYETLSLTA NFIIIIILCW GGYRVFTPRR NNVSHGDTRL ISQRIFWQIV FPATLFLILF QFAAFVGLLA SRENLVGVMP FNLGTLINYQ ALLVGNLIGV PLCYFIIRVV RNPFYLRSYY SQLKQQVDAK VTKKEFALWL LALGALLLLL CMPLNEKSTI FSTNYTLSLL LPLMMWGAMR YGYKLISLLW AVVLMISIHS YQNYIPIYPG YTTQLTITSS SYLVFSFIVN YMAVLATRQR AVVRRIQRLA YVDPVVHLPN VRALNRALRD APWSALCYLR IPGMEMLVKN YGIMLRIQYK QKLSHWLSPL LEPGEDVYQL SGNDLALRLN TESHQERITA LDSHLKQFRF FWDGMPMQPQ IGVSYCYVRS PVNHIYLLLG ELNTVAELSI VTNAPENMQR RGAMYLQREL KDKVAMMNRL QQALEHNHFF LMAQPITGMR GDVYHEILLR MKGENDELIS PDSFLPVAHE FGLSSSIDMW VIEHTLQFMA ENRAKMPAHR FAINLSPTSV CQARFPVEVS QLLAKYQIEA WQLIFEVTES NALTNVKQAQ ITLQHLQELG CQIAIDDFGT GYASYARLKN VNADLLKIDG SFIRNIVSNS LDYQIVASIC HLARMKKMLV VAEYVENEEI REAVLSLGID YMQGYLIGKP QPLIDTLNEI EPIRESA. It is sometimes possible for the material contained within the vial of "Cyclic di-GMP phosphodiesterase YfgF (yfgF), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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