Phosphodiesterase 10A Recombinant Protein | PDE10A recombinant protein

Recombinant Phosphodiesterase 10A (PDE10A)

Cat. Num. AAA2033021

• Gene Names: PDE10A; HSPDE10A
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Phosphodiesterase 10A; Recombinant Phosphodiesterase 10A (PDE10A); cAMP and cAMP-inhibited cGMP 3'; 5'-cyclic phosphodiesterase 10A; PDE10A recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: NYR RVPYHNWKHA VTVAHCMYAI LQNNHTLFTD LERKGLLIAC LCHDLDHRGF SNSYLQKFDH PLAALYSTST MEQHHFSQTV SILQLEGHNI FSTLSSSEYE QVLEIIRKAI IATDLALYFG NRKQLEEMYQ TGSLNLNNQS HRDRVIGLMM TACDLCSVTK LWPVTKLTAN DIYAEFWAEG DEMKKLGIQP IPMMDRDKKD EVPQGQLGFY NAVAIPCYTT LTQILPPTEPLLKACRDNLS QWEKVIRGEE TATWISSPSV AQKAAASED
• Sequence Length: 779

• Applicable Applications for PDE10A recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 34.7kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asn508~Asp779
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119567931
• NCBI GeneID: 10846
• Molecular Weight: 89,390 Da
• NCBI Official Full Name: phosphodiesterase 10A
• NCBI Official Synonym Full Names: phosphodiesterase 10A
• NCBI Official Symbol: PDE10A
• NCBI Official Synonym Symbols: HSPDE10A
• NCBI Protein Information: cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
• UniProt Protein Name: cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A
• Protein Family: cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE10A
• UniProt Entry Name: PDE10_HUMAN

NCBI Description

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase family. It plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This protein can hydrolyze both cAMP and cGMP to the corresponding nucleoside 5' monophosphate, but has higher affinity for cAMP, and is more efficient with cAMP as substrate. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

PDE10A: Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. Belongs to the cyclic nucleotide phosphodiesterase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; EC 3.1.4.17; Phosphodiesterase; EC 3.1.4.35

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: cytosol; membrane; perikaryon

Molecular Function: 3',5'-cyclic-GMP phosphodiesterase activity; 3',5'-cyclic-nucleotide phosphodiesterase activity; cAMP binding; cGMP binding; cGMP-stimulated cyclic-nucleotide phosphodiesterase activity; drug binding; metal ion binding

Biological Process: blood coagulation; cAMP catabolic process; cGMP catabolic process; signal transduction