PDCD1/PD1 recombinant protein

Recombinant PDCD1/PD1 (25-167) protein

Cat. Num. AAA389432

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Synonyms: PDCD1/PD1; Recombinant PDCD1/PD1 (25-167) protein; Programmed cell death protein 1; PDCD1/PD1 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant PDCD1/PD1 (25-167) protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant PDCD1/PD1 (25-167) protein that includes amino acids 25-167 of human PD1 protein (accession number NP_005009.2) was expressed in a baculovirus expression system, and contains an C-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant PDCD1 / PD1 (25-167) protein 12.5% SDS-PAGE Coomassie staining MW: 17.8 kDa Purity: >60%)

Related Product Information for PDCD1/PD1 recombinant protein

Background: Programmed cell death protein 1 (PDCD1), inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. PDCD1 delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2. Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. PDCD1 suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta. The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy.

Product Categories/Family for PDCD1/PD1 recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]