Western Blot (WB)
(Western Blot showing KLHL1 antibody used against human KLHL1 (AA: 202-506) recombinant protein. (Expected MW is 60.2 kDa))
Mouse KLHL1 Monoclonal Antibody | anti-KLHL1 antibody
KLHL1 antibody
Gene Names
KLHL1; MRP2
Applications
ELISA, Immunofluorescence, Western Blot
Synonyms
KLHL1; Monoclonal Antibody; KLHL1 antibody; Monoclonal KLHL1; Anti-KLHL1; KLHL 1; KLHL-1; Kelch like 1; Kelch-like protein 1; Mammalian Kelch like 1; anti-KLHL1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A8
Form/Format
Supplied as a liquid in ascitic fluid containing 0.03% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
748
Applicable Applications for anti-KLHL1 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
ELISA: 1:10,000
IF: 1:200-1:1000
WB: 1:200-1:1000
IF: 1:200-1:1000
WB: 1:200-1:1000
Biological Significance
KLHL1 was initially discovered as a homolog to the Drosophila Kelch gene that is highly expressed in several brain tissues. The predicted protein domain structure of KLHL1 is characteristic of a number of proteins that bind actin, form dimers, and often act as actin organizing proteins. Based on the presence of anti sense RNA that spans the transcription and translation start sites as well as the first splice site of KLHL1 in brain tissue of individuals suffering from the neurodegenerative disorder spinocerebellar ataxia type 8 (SCA8), it has been suggested that KLHL1 is involved in this disease and that regulation of KLHL1 protein may be affected by antisense RNA expression.
Cross-Reactivity
Human
Immunogen
KLHL1 antibody was raised in Mouse using a purified recombinant fragment of human KLHL1 expressed in E Coli as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot showing KLHL1 antibody used against human KLHL1 (AA: 202-506) recombinant protein. (Expected MW is 60.2 kDa))
Western Blot (WB)
(Western Blot showing KLHL1 antibody used against human KLHL1 (AA: 202-506) recombinant protein. (Expected MW is 60.2 kDa))
Related Product Information for anti-KLHL1 antibody
Mouse monoclonal KLHL1 antibody
Product Categories/Family for anti-KLHL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
82,666 Da
NCBI Official Full Name
kelch-like protein 1 isoform 1
NCBI Official Synonym Full Names
kelch-like family member 1
NCBI Official Symbol
KLHL1
NCBI Official Synonym Symbols
MRP2
NCBI Protein Information
kelch-like protein 1
UniProt Protein Name
Kelch-like 1 (Drosophila)
Protein Family
UniProt Gene Name
KLHL1
UniProt Entry Name
Q8TBJ7_HUMAN
NCBI Description
The KLHL1 protein belongs to a family of actin-organizing proteins related to Drosophila Kelch (Nemes et al., 2000 [PubMed 10888605]).[supplied by OMIM, Feb 2010]
Uniprot Description
KLHL1: May play a role in organizing the actin cytoskeleton of the brain cells.
Chromosomal Location of Human Ortholog: 13q21
Cellular Component: cytoskeleton; cell soma; cytoplasm; dendrite
Molecular Function: actin binding
Biological Process: dendrite development; cerebellar Purkinje cell layer development; actin cytoskeleton organization and biogenesis; adult walking behavior
Research Articles on KLHL1
Similar Products
Product Notes
The KLHL1 klhl1 (Catalog #AAA830205) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's KLHL1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). ELISA: 1:10,000 IF: 1:200-1:1000 WB: 1:200-1:1000. Researchers should empirically determine the suitability of the KLHL1 klhl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLHL1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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