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Human Prenylcysteine oxidase 1 ELISA Kit | PCYOX1 elisa kit

Human Prenylcysteine oxidase 1 (PCYOX1) ELISA Kit

Gene Names
PCYOX1; PCL1
Reactivity
Human
Synonyms
Prenylcysteine oxidase 1; Human Prenylcysteine oxidase 1 (PCYOX1) ELISA Kit; PCYOX1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PCYOX1. No significant cross-reactivity or interference between Human PCYOX1 and analogues was observed.
Sequence Length
505
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PCYOX1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PCYOX1 in samples. An antibody specific for PCYOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCYOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCYOX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCYOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,507 Da
NCBI Official Full Name
prenylcysteine oxidase 1
NCBI Official Synonym Full Names
prenylcysteine oxidase 1
NCBI Official Symbol
PCYOX1
NCBI Official Synonym Symbols
PCL1
NCBI Protein Information
prenylcysteine oxidase 1
UniProt Protein Name
Prenylcysteine oxidase 1
Protein Family
UniProt Gene Name
PCYOX1
UniProt Synonym Gene Names
KIAA0908; PCL1

NCBI Description

Prenylcysteine is released during the degradation of prenylated proteins. PCYOX1 catalyzes the degradation of prenylcysteine to yield free cysteines and a hydrophobic isoprenoid product (Tschantz et al., 1999 [PubMed 10585463]).[supplied by OMIM, Mar 2008]

Uniprot Description

PCYOX1: Involved in the degradation of prenylated proteins. Cleaves the thioether bond of prenyl-L-cysteines, such as farnesylcysteine and geranylgeranylcysteine. Belongs to the prenylcysteine oxidase family.

Protein type: EC 1.8.3.5; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: lysosome; plasma membrane; vacuolar membrane

Molecular Function: chloride-transporting ATPase activity; prenylcysteine oxidase activity

Biological Process: prenylated protein catabolic process; prenylcysteine catabolic process; prenylcysteine metabolic process

Research Articles on PCYOX1

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Product Notes

The Human PCYOX1 pcyox1 (Catalog #AAA281941) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281941 ELISA Kit recognizes Human PCYOX1. It is sometimes possible for the material contained within the vial of "Prenylcysteine oxidase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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