PCSK1 recombinant protein

PCSK1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120013

• Gene Names: PCSK1; PC1; PC3; NEC1; SPC3; BMIQ12; PCSK1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: PCSK1; PCSK1 Protein; Human; Recombinant (His Tag); Human PCSK1/NEC1 Protein (His Tag); BMIQ12 Protein; NEC1 Protein; PC1 Protein; PC3 Protein; SPC3 Protein; proprotein convertase subtilisin/kexin type 1; PCSK1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 25mM Tris, 150mM NaCl, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg617

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 111
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PCSK1 (NP_000430.3)(Met 1-Arg 617) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PCSK1 recombinant protein

Background: Neuroendocrine convertase 1, also known as Prohormone convertase 1, Proprotein convertase 1, PCSK1 and NEC1, is an enzyme which belongs to the peptidase S8 family and Furin subfamily. PCSK1 is an enzyme that performs the proteolytic cleavage of prohormones to their intermediate (or sometimes completely cleaved) forms. It is present only in neuroendocrine cells such as brain, pituitary and adrenal, and most often cleaves after a pair of basic residues within prohormones but can occasionally cleave after a single arginine. It binds to a protein known as proSAAS, which also represents its endogenous inhibitor. PCSK1 is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK1 substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency). PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction. It is due to impaired processing of prohormones.

Product Categories/Family for PCSK1 recombinant protein

Serine Proteases and Regulators

References

Jackson RS. et al., 2003, J. Clin. Invest. 112:1550-60. Farooqi I.S. et al., 2007, J. Clin. Endocrinol. Metab. 92: 3369-73. Benzinou,M. et al., 2008,Nat Genet. 40 (8):943-5. Benzinou M. et al., 2008, Nat. Genet. 40:943-5. Renstrom F, et al., 2009, Hum. Mol. Genet. 18 (8): 1489-96.

NCBI and Uniprot Product Information

• NCBI GI #: 20336242
• NCBI GeneID: 5122
• UniProt Accession #: P29120; P78478; Q92532
• NCBI Official Full Name: neuroendocrine convertase 1 isoform 1 preproprotein [Homo sapiens]
• NCBI Official Synonym Full Names: proprotein convertase subtilisin/kexin type 1
• NCBI Official Symbol: PCSK1
• NCBI Official Synonym Symbols: PC1; PC3; NEC1; SPC3; BMIQ12; PCSK1
• UniProt Protein Name: Neuroendocrine convertase 1
• Protein Family: Neuroendocrine convertase
• UniProt Gene Name: PCSK1
• UniProt Synonym Gene Names: NEC1; NEC 1; PC1
• UniProt Entry Name: NEC1_HUMAN

Uniprot Description

PCSK1: Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: EC 3.4.21.93; Protease

Chromosomal Location of Human Ortholog: 5q15-q21

Cellular Component: Golgi apparatus; extracellular space; transport vesicle

Molecular Function: serine-type endopeptidase activity

Biological Process: cellular protein metabolic process; cell-cell signaling; metabolic process; peptide hormone processing; proteolysis; regulation of insulin secretion; peptide biosynthetic process

Disease: Proprotein Convertase 1/3 Deficiency; Body Mass Index Quantitative Trait Locus 12