Mouse ProlifeRating Cell Nuclear Antigen ELISA Kit | PCNA elisa kit

Mouse ProlifeRating Cell Nuclear Antigen ELISA Kit

Cat. Num. AAA721493

• Gene Names: PCNA; ATLD2
• Reactivity: Mouse
• Synonyms: ProlifeRating Cell Nuclear Antigen; Mouse ProlifeRating Cell Nuclear Antigen ELISA Kit; PCNA elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PCNA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PCNA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PCNA elisa kit

Principle of the Assay: PCNA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PCNA antibody and an PCNA-HRP conjugate. The assay sample and buffer are incubated together with PCNA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PCNA concentration since PCNA from samples and PCNA-HRP conjugate compete for the anti-PCNA antibody binding site. Since the number of sites is limited, as more sites are occupied by PCNA from the sample, fewer sites are left to bind PCNA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PCNA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PCNA elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 49456555
• NCBI GeneID: 5111
• UniProt Accession #: Q6FHF5; P12004
• Molecular Weight: 28,706 Da
• NCBI Official Full Name: PCNA, partial
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Official Synonym Symbols: ATLD2
• NCBI Protein Information: proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: Proliferating cell nuclear antigen
• UniProt Gene Name: PCNA
• UniProt Entry Name: Q6FHF5_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: Cell cycle regulation; DNA replication

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: nucleoplasm; centrosome; PCNA complex; nuclear replication fork; cytoplasm; DNA replication factor C complex; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding

Biological Process: mismatch repair; positive regulation of deoxyribonuclease activity; heart development; DNA strand elongation during DNA replication; response to lipid; DNA repair; leading strand elongation; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; nucleotide-excision repair; base-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; nucleotide-excision repair, DNA gap filling; mitotic cell cycle; telomere maintenance; G1/S transition of mitotic cell cycle; regulation of DNA replication

Disease: Ataxia-telangiectasia-like Disorder 2