Phosphoenolpyruvate Carboxykinase 1 Recombinant Protein | PCK1 recombinant protein

Recombinant Human Phosphoenolpyruvate Carboxykinase 1

Cat. Num. AAA144719

• Gene Names: PCK1; PEPCK1; PEPCKC; PEPCK-C
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Phosphoenolpyruvate Carboxykinase 1; Recombinant Human Phosphoenolpyruvate Carboxykinase 1; PCK1 Human; Phosphoenolpyruvate Carboxykinase 1 Human Recombinant; Phosphoenolpyruvate carboxykinase 1 (soluble); PEPCK-C; PEPCK1; PEP carboxykinase; phosphoenolpyruvate carboxykinase cytosolic [GTP]; EC 4.1.1.32; PCK1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The PCK1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMPPQLQ NGLNLSAKVV QGSLDSLPQA VREFLENNAE LCQPDHIHIC DGSEEENGRL LGQMEEEGIL RRLKKYDNCW LALTDPRDVA RIESKTVIVT QEQRDTVPIP KTGLSQLGRW MSEEDFEKAF NARFPGCMKG RTMYVIPFSM GPLGSPLSKI GIELTDSPYV VASMRIMTRM GTPVLEALGD GEFVKCLHSV GCPLPLQKPL VNNWPCNPEL TLIAHLPDRR EIISFGSGYG GNSLLGKKCF ALRMASRLAK EEGWLAEHML VLGITNPEGE KKYLAAAFPS ACGKTNLAMM NPSLPGWKVE CVGDDIAWMK FDAQGHLRAI NPENGFFGVA PGTSVKTNPN AIKTIQKNTI FTNVAETSDG GVYWEGIDEP LASGVTITSW KNKEWSSEDG EPCAHPNSRF CTPASQCPII DAAWESPEGV PIEGIIFGGR RPAGVPLVYE ALSWQHGVFV GAAMRSEATA AAEHKGKIIM HDPFAMRPFF GYNFGKYLAH WLSMAQHPAA KLPKIFHVNW FRKDKEGKFL WPGFGENSRV LEWMFNRIDG KASTKLTPIG YIPKEDALNL KGLGHINMME LFSISKEFWE KEVEDIEKYL EDQVNADLPC EIEREILALK QRISQM.
• Sequence Length: 622

Related Product Information for PCK1 recombinant protein

Description: PCK1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 646 amino acids (1-622) and having a molecular mass of 71.7kDa.PCK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: PCK1 is a central regulating point for the regulation of gluconeogenesis. PCK1 has a vital part in this process by stimulating hepatic glucose production. The expression of PCK1 is controlled by insulin, cAMP, glucagon, glucocorticoids and diet. Modulation of the signals controlling PCK1 levels offers a possible therapeutic tactic to the treatment of Insulin resistance and subsequently obesity.

Product Categories/Family for PCK1 recombinant protein

Protein Kinases; Enzymes; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 187281517
• NCBI GeneID: 5105
• NCBI Accession #: NP_002582.3
• NCBI GenBank Nucleotide #: NM_002591.3
• UniProt Accession #: P35558; Q8TCA3; Q9UJD2; A8K437; B4DT64
• Molecular Weight: 34,156 Da
• NCBI Official Full Name: phosphoenolpyruvate carboxykinase, cytosolic
• NCBI Official Synonym Full Names: phosphoenolpyruvate carboxykinase 1 (soluble)
• NCBI Official Symbol: PCK1
• NCBI Official Synonym Symbols: PEPCK1; PEPCKC; PEPCK-C
• NCBI Protein Information: phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic [GTP]; PEP carboxykinase; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase
• UniProt Protein Name: Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
• Protein Family: Protein kinase
• UniProt Gene Name: PCK1
• UniProt Synonym Gene Names: PEPCK1; PEPCK-C
• UniProt Entry Name: PCKGC_HUMAN

NCBI Description

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

PCK1: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Lyase; Kinase, other; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - citrate (TCA) cycle; EC 4.1.1.32

Chromosomal Location of Human Ortholog: 20q13.31

Cellular Component: cytoplasm; cytosol

Molecular Function: GDP binding; GTP binding; carboxylic acid binding; manganese ion binding; phosphoenolpyruvate carboxykinase (GTP) activity; magnesium ion binding

Biological Process: oxaloacetate metabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; response to activity; glucose homeostasis; internal protein amino acid acetylation; glycerol biosynthetic process from pyruvate; drug metabolic process; response to insulin stimulus; gluconeogenesis

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Cytosolic