Human Polycomb group RING finger protein 2 ELISA Kit | PCGF2 elisa kit

Human Polycomb group RING finger protein 2 (PCGF2) ELISA Kit

Cat. Num. AAA280604

• Gene Names: PCGF2; MEL-18; RNF110; ZNF144
• Reactivity: Human
• Synonyms: Polycomb group RING finger protein 2; Human Polycomb group RING finger protein 2 (PCGF2) ELISA Kit; PCGF2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PCGF2. No significant cross-reactivity or interference between Human PCGF2 and analogues was observed.
• Sequence Length: 344

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PCGF2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PCGF2 in samples. An antibody specific for PCGF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCGF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCGF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCGF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 6005964
• NCBI GeneID: 7703
• NCBI Accession #: NP_009075.1
• NCBI GenBank Nucleotide #: NM_007144.2
• UniProt Accession #: P35227; A6NGD8
• Molecular Weight: 37,788 Da
• NCBI Official Full Name: polycomb group RING finger protein 2
• NCBI Official Synonym Full Names: polycomb group ring finger 2
• NCBI Official Symbol: PCGF2
• NCBI Official Synonym Symbols: MEL-18; RNF110; ZNF144
• NCBI Protein Information: polycomb group RING finger protein 2
• UniProt Protein Name: Polycomb group RING finger protein 2
• Protein Family: Polycomb group RING finger protein
• UniProt Gene Name: PCGF2
• UniProt Synonym Gene Names: MEL18; RNF110; ZNF144

NCBI Description

The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008]

Uniprot Description

PCGF2: Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero- posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Is not functionally redundant with Bmi1; unlike Bmi1 does not stimulate the E3 ubiquitin-protein ligase activity in a reconstituted PRC1-like complex.

Protein type: Transcription factor; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: nuclear body; nuclear chromatin; nucleoplasm; nucleus; PcG protein complex; sex chromatin

Molecular Function: chromatin binding; DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding

Biological Process: anterior/posterior pattern specification; embryonic skeletal morphogenesis; histone acetylation; in utero embryonic development; negative regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent