Mouse Cluster of Differentiation 68 ELISA Kit | CD68 elisa kit

Mouse Cluster of Differentiation 68 ELISA Kit

Cat. Num. AAA12240

• Gene Names: CD68; GP110; LAMP4; SCARD1
• Reactivity: Mouse
• Synonyms: Cluster of Differentiation 68; Mouse Cluster of Differentiation 68 ELISA Kit; CD68 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes recombinant and natural Mouse CD68, no significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Mouse CD68 in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Mouse CD68 concentration. The concentration of Mouse CD68 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD68 elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 298665
• NCBI GeneID: 968
• UniProt Accession #: P34810; Q53HR6; Q53XI3; Q96BI7; B4DVT4
• Molecular Weight: 37,408 Da
• NCBI Official Full Name: CD68
• NCBI Official Synonym Full Names: CD68 molecule
• NCBI Official Symbol: CD68
• NCBI Official Synonym Symbols: GP110; LAMP4; SCARD1
• NCBI Protein Information: macrosialin; CD68 antigen; macrophage antigen CD68; scavenger receptor class D, member 1
• UniProt Protein Name: Macrosialin
• Protein Family: Macrosialin
• UniProt Gene Name: CD68
• UniProt Entry Name: CD68_HUMAN

NCBI Description

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17p13

Cellular Component: membrane; lysosomal membrane; integral to membrane; plasma membrane; endosome membrane