Human Poly (rC)-binding protein 2 ELISA Kit | PCBP2 elisa kit

Human Poly (rC)-binding protein 2 (PCBP2) ELISA Kit

Cat. Num. AAA282190

• Gene Names: PCBP2; HNRPE2; HNRNPE2; hnRNP-E2
• Reactivity: Human
• Synonyms: Poly (rC)-binding protein 2; Human Poly (rC)-binding protein 2 (PCBP2) ELISA Kit; PCBP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human PCBP2. No significant cross-reactivity or interference between Human PCBP2 and analogues was observed.
• Sequence Length: 353

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PCBP2 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PCBP2 in samples. An antibody specific for PCBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 148833484
• NCBI GeneID: 5094
• NCBI Accession #: NP_001092090.1
• NCBI GenBank Nucleotide #: NM_001098620.2
• UniProt Accession #: Q15366; Q32Q82; Q59HD4; Q68Y55; Q6IPF4; Q6PKG5; A8K7X6; B4DXP5; F8VYL7; G3V0E8; I6L8F9
• Molecular Weight: 33,926 Da
• NCBI Official Full Name: poly(rC)-binding protein 2 isoform c
• NCBI Official Synonym Full Names: poly(rC) binding protein 2
• NCBI Official Symbol: PCBP2
• NCBI Official Synonym Symbols: HNRPE2; HNRNPE2; hnRNP-E2
• NCBI Protein Information: poly(rC)-binding protein 2
• UniProt Protein Name: Poly(rC)-binding protein 2
• Protein Family: Poly(rC)-binding protein
• UniProt Gene Name: PCBP2
• UniProt Synonym Gene Names: hnRNP E2

NCBI Description

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2018]

Uniprot Description

hnRNP E2: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytoplasm; cytosol; extracellular exosome; focal adhesion; intracellular ribonucleoprotein complex; membrane; nucleoplasm; nucleus

Molecular Function: DNA binding; enzyme binding; protein binding; RNA binding; ubiquitin protein ligase binding

Biological Process: defense response to virus; innate immune response; mRNA metabolic process; mRNA splicing, via spliceosome; negative regulation of defense response to virus; negative regulation of type I interferon production; proteasomal ubiquitin-dependent protein catabolic process; RNA metabolic process