PCBP1 recombinant protein

Recombinant Human PCBP1 Protein (His tag)

Cat. Num. AAA2545717

• Gene Names: PCBP1; HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
• Purity: > 87 % as determined by SDS-PAGE
• Synonyms: PCBP1; Recombinant Human PCBP1 Protein (His tag); HNRPE1; HNRPX; hnRNP-E1; hnRNP-X; PCBP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 87 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 500mM NaCl, 20% glycerol, pH 8.0
• Sequence Length: 356

• Application Notes: The recombinant human PCBP1 comprises 362 amino acids and has a predicted molecular mass of 38.3 kDa. It migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PCBP1 recombinant protein

Background: Poly (rC)-binding protein 1, also known as Heterogeneous nuclear ribonucleoprotein E1, Alpha-CP1, Nucleic acid-binding protein SUB2.3 and PCBP1, is a family member of heterogeneous nuclear ribonucleoproteins (hnRNPs) that belong to RNA-binding proteins and bear three KH domains. PCBP1 is loosely bound in the nucleus. It may shuttle between the nucleus and the cytoplasm. It is abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands. PCBP1 is widely expressed in many human tissues and involved in regulation of transcription, transportation process, and function of RNA molecules. PCBP1 plays a pivotal role in post-transcriptional regulation for RNA metabolism and RNA function in gene expression. PCBP1 acts as a negative regulator of CD44 variants splicing in HepG2 cells, and loss of PCBP1 in human hepatic tumor contributes to the formation of a metastatic phenotype .

Description: A DNA sequence encoding the human PCBP1 (NP_006187.2) (Asp 2-Cys 356) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 222352151
• NCBI GeneID: 5093
• NCBI Accession #: NP_006187.2
• NCBI GenBank Nucleotide #: NM_006196.3
• UniProt Accession #: Q15365; Q13157; Q14975
• Molecular Weight: 37,498 Da
• NCBI Official Full Name: poly(rC)-binding protein 1
• NCBI Official Synonym Full Names: poly(rC) binding protein 1
• NCBI Official Symbol: PCBP1
• NCBI Official Synonym Symbols: HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1
• NCBI Protein Information: poly(rC)-binding protein 1
• UniProt Protein Name: Poly(rC)-binding protein 1
• Protein Family: Poly(rC)-binding protein
• UniProt Gene Name: PCBP1
• UniProt Synonym Gene Names: hnRNP E1

NCBI Description

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]

Uniprot Description

hnRNP E1: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.

Protein type: RNA splicing; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 2p13.3

Cellular Component: cell-cell adherens junction; cytoplasm; intracellular membrane-bound organelle; membrane; nucleoplasm

Molecular Function: mRNA binding; protein binding; RNA binding; single-stranded DNA binding

Biological Process: gene expression; nuclear mRNA splicing, via spliceosome