Human Poly [ADP-ribose] polymerase 15 ELISA Kit | PARP15 elisa kit

Human Poly [ADP-ribose] polymerase 15 (PARP15) ELISA Kit

Gene Names

PARP15; BAL3; ARTD7; pART7

Reactivity

Human

Synonyms

Poly [ADP-ribose] polymerase 15; Human Poly [ADP-ribose] polymerase 15 (PARP15) ELISA Kit; PARP15 elisa kit

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Cat. Num. AAA281825 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of Human PARP15. No significant cross-reactivity or interference between Human PARP15 and analogues was observed.

Samples

Serum, Plasma, Other biological fluids

Assay Type

Sandwich

Sample Volume

1-200 uL

Precision

Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV

Detection Wavelength

450 nm

Preparation and Storage

Store at 2-8 degree C.

Related Product Information for PARP15 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PARP15 in samples. An antibody specific for PARP15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARP15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARP15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARP15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #

165377137

NCBI GeneID

165631

NCBI Accession #

NP_001106995.1

NCBI GenBank Nucleotide #

NM_001113523.2

UniProt Accession #

Q460N3; Q8N1K3; J3KR47

Molecular Weight

49,501 Da

NCBI Official Full Name

poly

NCBI Official Synonym Full Names

poly(ADP-ribose) polymerase family member 15

NCBI Official Symbol

PARP15

NCBI Official Synonym Symbols

BAL3; ARTD7; pART7

NCBI Protein Information

poly [ADP-ribose] polymerase 15

UniProt Protein Name

Poly [ADP-ribose] polymerase 15

Protein Family

Poly [ADP-ribose] polymerase

UniProt Gene Name

PARP15

UniProt Synonym Gene Names

BAL3; PARP-15; ARTD7

NCBI Description

PARP15 is a macrodomain-containing transcriptional repressor with poly(ADP-ribose) polymerase activity (Aguiar et al., 2005 [PubMed 16061477]).[supplied by OMIM, May 2008]

Uniprot Description

PARP15: Transcriptional repressor. Has ADP-ribosyltransferase activity. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.4.2.30; Transferase

Chromosomal Location of Human Ortholog: 3q21.1

Cellular Component: nucleus

Molecular Function: NAD+ ADP-ribosyltransferase activity; protein binding

Biological Process: negative regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent

Research Articles on PARP15

1. The current study investigated the possible association between PARP15 single-nucleotide polymorphisms and the risk of acute myeloid leukemia (AML). The results from a Cox regression analysis for overall survival revealed that two polymorphisms associated with increased overall survival and the signal for rs17208928 was retained after correcting for multiple tests. rs17208928 showed a significant association with survival

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Reactivity: n/a

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Reactivity: Human; other species are not tested.

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Product Notes

The Human PARP15 parp15 (Catalog #AAA281825) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281825 ELISA Kit recognizes Human PARP15. It is sometimes possible for the material contained within the vial of "Poly [ADP-ribose] polymerase 15, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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