PAPPA2 / Pappalysin 2 Recombinant Protein | PAPPA2 recombinant protein

Recombinant Human PAPPA2 / Pappalysin 2 Protein (His tag)

Cat. Num. AAA2546200

• Gene Names: PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: PAPPA2 / Pappalysin 2; Recombinant Human PAPPA2 / Pappalysin 2 Protein (His tag); RP4-774I24.1; PAPP-A2; PAPPE; PLAC3; PAPPA2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1791

• Application Notes: The secreted recombinant human PAPPA2 consists of 1174 amino acids with the predicted molecular mass of 131 kDa. As a result of glycosylation, rhPAPPA2 migrates as an approximately 170-180 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ser 234
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for PAPPA2 recombinant protein

Background: Pappalysin-2/PAPP-A2 is the second member of the pappalysin family of metzincin superfamily, of which PAPP-A is the first member. There is no homology between the prepro-peptides of PAPP-A and PAPP-A2, but 46% of the residues of mature PAPP-A are also present in mature PAPP-A2. PAPP-A specifically cleaves insulin-like growth factor-binding protein (IGFBP)-4, one of six known modulators of IGF-I and -II, whereas PAPP-A2 specifically cleaved IGFBP-5 at one site, between Ser-143 and Lys-144. In contrast to the cleavage of IGFBP-4 by PAPP-A that strictly requires the presence of IGF, the cleavage of IGFBP-5 by PAPP-A2 was IGF-independent. Recent data firmly establish PAPP-A and IGFBP-4 as an important functional pair in several systems. Because of its close relationship with PAPP-A, both structurally and functionally, PAPP-A2 is a likely candidate for IGFBP-5 proteinase in many tissues and conditioned media where IGFBP-5 proteolysis has been reported.

Description: A DNA sequence encoding the human PAPPA2 mature form (NP_064714.2) corresponding to amino acid (Ser 234-Cys 1396) was expressed, with a carboxy-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 116174738
• NCBI GeneID: 60676
• NCBI Accession #: NP_064714.2
• NCBI GenBank Nucleotide #: NM_020318.2
• UniProt Accession #: Q9BXP8; Q96PH7; Q96PH8; Q9H4C9; A9Z1Y8
• Molecular Weight: 92,136 Da
• NCBI Official Full Name: pappalysin-2 isoform 1
• NCBI Official Synonym Full Names: pappalysin 2
• NCBI Official Symbol: PAPPA2
• NCBI Official Synonym Symbols: PAPPE; PLAC3; PAPP-E; PAPP-A2
• NCBI Protein Information: pappalysin-2
• UniProt Protein Name: Pappalysin-2
• Protein Family: Pappalysin
• UniProt Gene Name: PAPPA2
• UniProt Synonym Gene Names: PLAC3; PAPP-A2; PAPP-E

NCBI Description

This gene encodes a member of the pappalysin family of metzincin metalloproteinases. The encoded protein cleaves insulin-like growth factor-binding protein 5 and is thought to be a local regulator of insulin-like growth factor (IGF) bioavailability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]

Uniprot Description

PAPPA2: Metalloproteinase which specifically cleaves IGFBP-5. Shows limited proteolysis toward IGFBP-3. Belongs to the peptidase M43B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q25.2

Cellular Component: extracellular region

Molecular Function: metalloendopeptidase activity; metallopeptidase activity

Biological Process: cellular protein metabolic process; proteolysis