Mouse Myosin-11 (MYH11) ELISA Kit | MYH11 elisa kit

Mouse Myosin-11 (MYH11) ELISA Kit

Cat. Num. AAA7236295

• Gene Names: Myh11; SM1; SM2; smMHC; AV071570
• Reactivity: Mouse
• Synonyms: Myosin-11 (MYH11); Mouse Myosin-11 (MYH11) ELISA Kit; MYH11 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This MYO-11 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MYO-11. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYH11 elisa kit

Principle of the assay: MYO-11 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MYO-11 antibody and an MYO-11-HRP conjugate. The assay sample and buffer are incubated together with MYO-11-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYO-11 concentration since MYO-11 from samples and MYO-11-HRP conjugate compete for the anti-MYO-11 antibody binding site. Since the number of sites is limited, as more sites are occupied by MYO-11 from the sample, fewer sites are left to bind MYO-11-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYO-11 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MYH11 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 241982718
• NCBI GeneID: 17880
• NCBI Accession #: NP_001155247.1
• NCBI GenBank Nucleotide #: NM_001161775.1
• Molecular Weight: 223,266 Da
• NCBI Official Full Name: myosin-11 isoform 2
• NCBI Official Synonym Full Names: myosin, heavy polypeptide 11, smooth muscle
• NCBI Official Symbol: Myh11
• NCBI Official Synonym Symbols: SM1; SM2; smMHC; AV071570
• NCBI Protein Information: myosin-11; myosin heavy chain 11, smooth muscle; myosin heavy chain, smooth muscle isoform
• UniProt Protein Name: Myosin-11
• Protein Family: Myosin
• UniProt Gene Name: Myh11
• UniProt Entry Name: MYH11_MOUSE

Uniprot Description

MYH11: Muscle contraction. A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11. Defects in MYH11 are the cause of familial aortic aneurysm thoracic type 4 (AAT4); also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Motor; Motility/polarity/chemotaxis

Cellular Component: smooth muscle contractile fiber; cytoplasm; stress fiber; muscle myosin complex; myosin complex

Molecular Function: calmodulin binding; structural constituent of muscle; motor activity; nucleotide binding; actin binding; ATP binding

Biological Process: elastic fiber assembly; smooth muscle contraction; cardiac muscle fiber development