Human Platelet Antibodies IgA ELISA Kit | PA-IgA elisa kit

Human Platelet Antibodies IgA ELISA Kit

Cat. Num. AAA37576

• Gene Names: Prdm16; csp1; mel1; 5730557K01Rik
• Reactivity: Human
• Synonyms: Platelet Antibodies IgA; Human Platelet Antibodies IgA ELISA Kit; PA-IgA elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human PA-IGA and analogues was observed.
• Sequence Length: 1178

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 0.25-8ng/ml.
• Sensitivity: 0.1ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PA-IGA concentrations in Human serum, plasma and other body fluids. Using Purified Human PA-IGA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PA-IGA and PA-IGA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PA-IGA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PA-IgA elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PA-IGA concentrations in Human serum, plasma and other body fluids. Using Purified Human PA-IGA antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PA-IGA and PA-IGA antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PA-IGA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 597517970
• NCBI GeneID: 70673
• NCBI Accession #: NP_001277958.1
• NCBI GenBank Nucleotide #: NM_001291029.1
• UniProt Accession #: A2A935; Q69ZD6; Q6PB79; Q7TPF4
• Molecular Weight: 140,858 Da
• NCBI Official Full Name: PR domain zinc finger protein 16 isoform 4
• NCBI Official Synonym Full Names: PR domain containing 16
• NCBI Official Symbol: Prdm16
• NCBI Official Synonym Symbols: csp1; mel1; 5730557K01Rik
• NCBI Protein Information: PR domain zinc finger protein 16; line 27; MDS1/EVI1-like gene 1; transcription factor MEL1; PR domain-containing protein 16
• UniProt Protein Name: PR domain zinc finger protein 16
• UniProt Gene Name: Prdm16
• UniProt Synonym Gene Names: Kiaa1675; Mel1; MDS1/EVI1-like gene 1
• UniProt Entry Name: PRD16_MOUSE

Uniprot Description

PRDM16: binds DNA and functions as a transcriptional regulator. A repressor of TGF-beta signaling. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Interacts with HDAC1, SKI, SMAD2 and SMAD3; the interaction with SKI promotes the recruitment of SMAD3-HDAC1 complex on the promoter of TGF-beta target genes. A chromosomal aberration involving PRDM16 is found in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). Reciprocal translocation t(1;3)(p36;q21). Isoform 4 is specifically expressed in adult T-cell leukemia. Four human isoforms are produced by alternative promoter usage and alternative splicing

Protein type: Methyltransferase, protein lysine, predicted; C2H2-type zinc finger protein

Cellular Component: transcriptional repressor complex; nucleus

Molecular Function: protein binding; DNA binding; nucleic acid binding; transcription activator binding; sequence-specific DNA binding; metal ion binding; transcription coactivator activity; SMAD binding

Biological Process: tongue development; transcription, DNA-dependent; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; palate development; negative regulation of granulocyte differentiation; regulation of cellular respiration; neurogenesis; regulation of transcription, DNA-dependent; white fat cell differentiation; brown fat cell differentiation; cell differentiation; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway