p38 MAPK Phospho-Regulation Antibody Sampler Kit | p38K antibody sampler kit
p38 MAPK Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA, Immunocytochemistry
Synonyms
p38 MAPK Phospho-Regulation; p38K antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for p38K antibody sampler kit
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for p38K antibody sampler kit
Kit Summary: The p38 phospho-regulation antibody sampler kit can be used to detect p38 phosphorylation at Thr-180/Tyr-182 and Tyr-323. The kit also includes an antibody to examine total p38 expression levels and secondary reagents for rabbitpolyclonal and mouse monoclonal antibody detection.
Background: p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase.This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Fourisoforms of p38 MAPK (alpha, beta, gamma, delta) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by avariety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growthfactors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has beenshown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, andMEF2. T cells possess an alternative pathway for p38alpha activation where stimulation of the antigen receptor (TCR)induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 andcatalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.
Background: p38 MAP kinase (MAPK), also called RK, CSBP, and SAPK2a, is the mammalian orthologue of the yeast HOG kinase.This family of kinases participates in signaling cascades that control cellular responses to cytokines and stress. Fourisoforms of p38 MAPK (alpha, beta, gamma, delta) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by avariety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides, UV light, and growthfactors. MKK3 and SEK activate p38 MAPK by dual phosphorylation at Thr-180/Tyr-182. Activated p38 MAPK has beenshown to phosphorylate and activate MAPKAP kinase 2 and to phosphorylate the transcription factors ATF-2, Max, andMEF2. T cells possess an alternative pathway for p38alpha activation where stimulation of the antigen receptor (TCR)induces phosphorylation of p38 on Tyr-323. This site is required for TCR-mediated phosphorylation of Thr-180 andcatalytic activity. Thus, Tyr-323 may also have important roles in regulating p38 MAP kinase pathways.
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Product Notes
The Human, Rat, Mouse p38K (Catalog #AAA474488) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474488 Antibody Sampler Kit recognizes Human, Rat, Mouse p38K. AAA Biotech's p38 MAPK Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the p38K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p38 MAPK Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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