Toxoplasma Gondii SAG1 protein Recombinant Protein | TOXGO SAG1 recombinant protein

Toxoplasma Gondii SAG1 protein Recombinant

Cat. Num. AAA553063

• Applications: ELISA
• Purity: GST affinity chromatography; Purity: >90%
• Synonyms: Toxoplasma Gondii SAG1 protein; Toxoplasma Gondii SAG1 protein Recombinant; toxoplasma gondii; sag1; p30; TOXGO SAG1 recombinant protein

• Host: E Coli
• Purity/Purification: GST affinity chromatography; Purity: >90%
• Form/Format: 0.2um filtered solution: 50mM Tris-HCL pH 8.0, 60mM NaCl, 0.25% Sarkosyl, 10mM glutathione, 50% glycerol.
• Concentration: 12.8 mg/ml (varies by lot)
• Sequence Length: 336

• Applicable Applications for TOXGO SAG1 recombinant protein: ELISA
• Application Notes: Suitable for use in ELISA
• Host Note: DNA sequence encoding immunodominant fragment of Toxoplasma SAG1 (p30) protein was expressed in Escherichia Coli.
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Recombinant TOXGO SAG1 (p30) encodes the immunodominant sequences of the Toxoplasma Dense Granule protein.; ; Recombinant SAG1 contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: This protein can be stored in working aliquots at 2 - 8 degree C for one month, or at -20 degree C for six months without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Related Product Information for TOXGO SAG1 recombinant protein

Recent studies indicate that SAG1 may play an important role in invasion of host cells. Unlike surface antigens of other protozoa, such as plasmodia and trypanosomes, SAG1 is a nonvariant antigen which is well conserved immunologically and at the sequence level. SAG1 elicits a robust immune response in infected animals, including humans, and immunization with purified SAG1 protects mice from a lethal parasite challenge. This immunity appears to be primarily mediated by CD8+ cells specific for SAG1

Product Categories/Family for TOXGO SAG1 recombinant protein

Viral Antigens

References

• evaluation of nested pcr targeting b1 gene with four npcrs (targeting the b1, sag2 and SAG1 gene) for detection of toxoplasma gondii genome in intra ocular fluids from toxoplasma retinochoroiditis patients in a tertiary eye hospital, chennai, india .Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 857.
• reverse enzyme-linked immunosorbent assay using monoclonal antibodies against SAG1-related sequence, sag2a, and p97 antigens from toxoplasma gondii to detect specific immunoglobulin g (igg), igm, and iga antibodies in human sera .Clin. Vaccine Immunol., Aug 2008; 15: 1265 - 1271.
• azurin-like protein blocks invasion of toxoplasma gondii through potential interactions with parasite surface antigen SAG1 .Antimicrob. Agents Chemother., Feb 2008; 52: 402 - 408.
• the SAG1 toxoplasma gondii surface protein is not required for acute ocular toxoplasmosis in mice .Infect. Immun., Apr 2007; 75: 2079 - 2083.
• conformationally correct expression of membrane-anchored toxoplasma gondii SAG1 in the primitive protozoan giardia duodenalis .Infect. Immun., Feb 2002; 70: 1014 - 1016.