OXSR1 / OSR1 recombinant protein

Recombinant Human OXSR1 / OSR1 Protein (GST tag)

Cat. Num. AAA2545091

• Gene Names: OXSR1; OSR1
• Purity: > 88 % as determined by SDS-PAGE
• Synonyms: OXSR1 / OSR1; Recombinant Human OXSR1 / OSR1 Protein (GST tag); KIAA1101; OSR1; OXSR1; OXSR1 / OSR1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 88 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.5mM GSH, 0.5mM PMSF, 0.5mM EDTA, 10% glycerol
• Sequence Length: 527

• Application Notes: The recombinant human GST/OXSR1 chimera consists of 752 amino acids and predicts a molecular mass of 84 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for OXSR1 / OSR1 recombinant protein

Background: Oxidative stress-responsive 1 protein (OXSR1), also known as Serine/threonine-protein kinase OSR1, is a member of the Ser/Thr protein kinase family of proteins. OXSR1 regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. OXSR1 is a 58 kDa protein of 527 amino acids that is widely expressed in mammalian tissues and cell lines. The amino acid (aa) sequence of the predicted OXSR1 protein is 39% identical to that of human SOK1. Of potential regulators surveyed, endogenous OXSR1 is activated only by osmotic stresses, notably sorbitol and to a lesser extent NaCl. OXSR1 did not increase the activity of coexpressed JNK, nor did it activate three other MAPKs, p38, ERK2, and ERK5. Phosphorylation by OXSR1 modulates the G protein sensitivity of PAK isoforms. The OXSR1 and SPAK are key enzymes in a signalling cascade regulating the activity of Na+/K+/2Cl- co-transporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which recognizes a unique peptide (Arg-Phe-Xaa-Val) motif. The OXSR1 and SPAK kinases specifically recognize their upstream activators and downstream substrates.

Description: A DNA sequence encoding the full length of human OXSR1 (NP_005100.1) (Met 1-Ser 527) was fused with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 4826878
• NCBI GeneID: 9943
• NCBI Accession #: NP_005100.1
• NCBI GenBank Nucleotide #: NM_005109.2
• UniProt Accession #: O95747; Q3LR53; Q7Z501; Q9UPQ1
• Molecular Weight: 58,022 Da
• NCBI Official Full Name: serine/threonine-protein kinase OSR1
• NCBI Official Synonym Full Names: oxidative stress responsive 1
• NCBI Official Symbol: OXSR1
• NCBI Official Synonym Symbols: OSR1
• NCBI Protein Information: serine/threonine-protein kinase OSR1
• UniProt Protein Name: Serine/threonine-protein kinase OSR1
• UniProt Gene Name: OXSR1

NCBI Description

The product of this gene belongs to the Ser/Thr protein kinase family of proteins. It regulates downstream kinases in response to environmental stress, and may play a role in regulating the actin cytoskeleton. [provided by RefSeq, Jul 2008]

Uniprot Description

OSR1: oxidative-stress responsive 1 protein is a ubiquitously expressed protein kinase of the STE20 family. Regulates Na-K-Cl cotransporter (NKCC) activity. Regulates downstream kinases in response to environmental stress. May also have a function in regulating the actin cytoskeleton. Binds to and phosphorylates PAK1. Interacts and is phosphorylated by WNK1. Knockdown of either WNK1 or OXSR1 reduces NKCC activity. Interacts with chloride channel proteins SLC12A6 isoform 2, SLC12A1 and SLC12A2 but not with SLC12A4 and SLC12A7, possibly establishing sensor/signaling modules that initiate the cellular response to environmental stress. An important paralog of this gene is STRADB.

Protein type: EC 2.7.11.1; FRAY subfamily; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family

Chromosomal Location of Human Ortholog: 3p22.2

Cellular Component: cytoplasm

Molecular Function: ATP binding; magnesium ion binding; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-threonine phosphorylation; protein amino acid phosphorylation