OTUB1 recombinant protein

Recombinant Human OTUB1 protein

Cat. Num. AAA1562046

• Gene Names: OTUB1; OTB1; OTU1; HSPC263
• Purity: >90% as determined by SDS-PAGE
• Synonyms: OTUB1; Recombinant Human OTUB1 protein; Ubiquitin thioesterase OTUB1; Deubiquitinating enzyme OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Otubain-1; hOTU1; Ubiquitin-specific-processing protease OTUB1; OTUB1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human OTUB1 (Met1-Lys271) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for OTUB1 recombinant protein

Ubiquitin thioesterase OTUB1, also known as Deubiquitinating enzyme OTUB1, OTU domain-containing ubiquitin aldehyde-binding protein 1, Otubain-1, Ubiquitin-specific-processing protease OTUB1, OTUB1 and OTB1, is a cytoplasm protein which belongs to thepeptidase C65 family. OTUB1 is a hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. OTUB1 is a regulator of T-cell anergy, a phenomenon that occurs when T-cells are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. OTUB1 acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Isoform1 of OTUB1 destabilizes RNF128, leading to prevent anergy. In contrast, isoform2of OTUB1 stabilizes RNF128 and promotes anergy. OTUB1 regulates RNF128-mediated ubiquitination, but does not deubiquitinate polyubiquitinated RNF128. Deubiquitinates estrogen receptor alpha (ESR1). OTUB1 mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains, but not 'Lys-63'-linked polyubiquitin chains. OTUB1 is also capable of removing NEDD8 from NEDD8 conjugates, but with a much lower preference compared to 'Lys-48'-linked ubiquitin.

Product Categories/Family for OTUB1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 109148508
• NCBI GeneID: 55611
• NCBI Accession #: NP_060140.2
• NCBI GenBank Nucleotide #: NM_017670.2
• UniProt Accession #: Q96FW1; Q32Q78; Q96II3; Q9NXQ4; Q9P0B8
• Molecular Weight: 35,285 Da
• NCBI Official Full Name: ubiquitin thioesterase OTUB1
• NCBI Official Synonym Full Names: OTU deubiquitinase, ubiquitin aldehyde binding 1
• NCBI Official Symbol: OTUB1
• NCBI Official Synonym Symbols: OTB1; OTU1; HSPC263
• NCBI Protein Information: ubiquitin thioesterase OTUB1; OTU domain, ubiquitin aldehyde binding 1; OTU domain-containing ubiquitin aldehyde-binding protein 1; OTU-domain Ubal-binding 1; deubiquitinating enzyme OTUB1; otubain-1; ubiquitin-specific protease otubain 1; ubiquitin-speci
• UniProt Protein Name: Ubiquitin thioesterase OTUB1
• Protein Family: Ubiquitin thioesterase
• UniProt Gene Name: OTUB1
• UniProt Synonym Gene Names: OTB1; OTU1; hOTU1
• UniProt Entry Name: OTUB1_HUMAN

NCBI Description

The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

OTUB1: a protease that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occur when T-cell are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Two alternatively spliced isoforms are known.

Protein type: Ubiquitin-specific protease; EC 3.4.19.12; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q13.1

Cellular Component: cytoplasm

Molecular Function: protein binding; ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity; NEDD8-specific protease activity

Biological Process: immune system process; proteolysis; DNA repair; response to DNA damage stimulus