Otubain 1 (OTUB1) Recombinant Protein | OTUB1 recombinant protein

Recombinant Otubain 1 (OTUB1)

Cat. Num. AAA2011421

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Otubain 1 (OTUB1); Recombinant Otubain 1 (OTUB1); OTUB1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal His-tag, its sequence is listed below.; MGHHHHHHSGS-MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKE YAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGF TEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K
• Sequence Length: 271

• Applicable Applications for OTUB1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Lys271 (Accession # B2RYG6) with a N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 157821731
• NCBI GeneID: 293705
• NCBI Accession #: NP_001099802.1
• NCBI GenBank Nucleotide #: NM_001106332.1
• UniProt Accession #: B2RYG6
• Molecular Weight: 32.5kDa
• NCBI Official Full Name: ubiquitin thioesterase OTUB1
• NCBI Official Synonym Full Names: OTU domain, ubiquitin aldehyde binding 1
• NCBI Official Symbol: Otub1
• NCBI Protein Information: ubiquitin thioesterase OTUB1; otubain-1; deubiquitinating enzyme OTUB1; ubiquitin-specific-processing protease OTUB1; OTU domain-containing ubiquitin aldehyde-binding protein 1
• UniProt Protein Name: Ubiquitin thioesterase OTUB1
• Protein Family: Ubiquitin thioesterase
• UniProt Gene Name: Otub1
• UniProt Entry Name: OTUB1_RAT

Uniprot Description

OTUB1: a protease that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Regulator of T-cell anergy, a phenomenon that occur when T-cell are rendered unresponsive to antigen rechallenge and no longer respond to their cognate antigen. Acts via its interaction with RNF128/GRAIL, a crucial inductor of CD4 T-cell anergy. Two alternatively spliced isoforms are known.

Protein type: Protease; EC 3.4.19.12; Ubiquitin conjugating system; Ubiquitin-specific protease

Cellular Component: cytoplasm; nucleus

Molecular Function: ubiquitin binding; ubiquitin protein ligase binding; ubiquitin-specific protease activity; NEDD8-specific protease activity

Biological Process: immune system process; DNA repair; proteolysis; response to DNA damage stimulus