Human Mu-type opioid receptor ELISA Kit | OPRM1 elisa kit

Human Mu-type opioid receptor ELISA Kit

Cat. Num. AAA764255

• Gene Names: Oprm1; MORA; Oprm; Oprrm1
• Reactivity: Human
• Synonyms: Mu-type opioid receptor; Human Mu-type opioid receptor ELISA Kit; OPRM1 (Mu-type opioid receptor)/LMOR/MOP/MOR/MOR1/hMOP/M-OR-1/MOR-1/Mu opiate receptor/opioid receptor; mu 1/OPRM; OPRM1 elisa kit

• Reactivity: Human
• Sequence Length: 393

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for OPRM1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human OPRM1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human OPRM1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human OPRM1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman OPRM1can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 145553980
• NCBI GeneID: 25601
• NCBI Accession #: NP_001033688.2
• NCBI GenBank Nucleotide #: NM_001038599.2
• Molecular Weight: 43,317 Da
• NCBI Official Full Name: mu-type opioid receptor isoform 1B2
• NCBI Official Synonym Full Names: opioid receptor, mu 1
• NCBI Official Symbol: Oprm1
• NCBI Official Synonym Symbols: MORA; Oprm; Oprrm1
• NCBI Protein Information: mu-type opioid receptor
• UniProt Protein Name: Mu-type opioid receptor
• Protein Family: Mu-type opioid receptor
• UniProt Gene Name: Oprm1
• UniProt Synonym Gene Names: Ror-b; M-OR-1; MOR-1
• UniProt Entry Name: OPRM_RAT

NCBI Description

receptor that binds morphine with high affinity [RGD, Feb 2006]

Uniprot Description

MOR-1: a Gi-protein-coupled receptor for beta-endorphin, morphine and other opiates. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Ligand-binding inactivates adenylyl cyclase, and activates a variety of G-beta-gamma-dependent pathways including the MAPK and the PI3K/Akt cascades. Two splice-variant isoforms have been described.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: cytoplasm; cytosol; dendrite; dendrite cytoplasm; focal adhesion; integral to membrane; integral to plasma membrane; lipid raft; membrane; perikaryon; sarcolemma

Molecular Function: beta-endorphin receptor activity; filamin binding; G-protein alpha-subunit binding; G-protein beta-subunit binding; G-protein coupled receptor activity; neuropeptide binding; opioid receptor activity; protein binding; protein C-terminus binding; protein domain specific binding; voltage-gated calcium channel activity

Biological Process: acute inflammatory response to antigenic stimulus; behavioral response to ethanol; dopamine receptor, adenylate cyclase activating pathway; eating behavior; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase inhibiting pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; locomotory behavior; negative regulation of adenylate cyclase activity; negative regulation of cAMP biosynthetic process; negative regulation of nitric oxide biosynthetic process; neuropeptide signaling pathway; opioid receptor, adenylate cyclase inhibiting pathway; positive regulation of appetite; positive regulation of neurogenesis; positive regulation of nitric oxide biosynthetic process; reduction of cytosolic calcium ion concentration; regulation of excitatory postsynaptic membrane potential; regulation of sensory perception of pain; response to cocaine; response to ethanol; response to food; response to lipopolysaccharide; response to morphine; response to radiation; sensory perception of pain; wound healing