Melanopsin Blocking Peptide | OPN4 blocking peptide

Melanopsin Blocking Peptide

Cat. Num. AAA9622318

• Gene Names: OPN4; MOP
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: Melanopsin; Melanopsin Blocking Peptide; OPN4 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for OPN4 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for OPN4 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 71999143
• NCBI GeneID: 94233
• NCBI Accession #: NP_001025186.1
• NCBI GenBank Nucleotide #: NP_001025186.1
• UniProt Accession #: Q9UHM6; Q14D01; Q2PP22; Q8NGQ9; B7ZLB3
• Molecular Weight: 52,635 Da
• NCBI Official Full Name: melanopsin isoform 2
• NCBI Official Synonym Full Names: opsin 4
• NCBI Official Symbol: OPN4
• NCBI Official Synonym Symbols: MOP
• NCBI Protein Information: melanopsin; opsin-4
• UniProt Protein Name: Melanopsin
• Protein Family: Melanopsin
• UniProt Gene Name: OPN4
• UniProt Synonym Gene Names: MOP
• UniProt Entry Name: OPN4_HUMAN

NCBI Description

Opsins are members of the guanine nucleotide-binding protein (G protein)-coupled receptor superfamily. This gene encodes a photoreceptive opsin protein that is expressed within the ganglion and amacrine cell layers of the retina. In mouse, retinal ganglion cell axons expressing this gene projected to the suprachiasmatic nucleus and other brain nuclei involved in circadian photoentrainment. In mouse, this protein is coupled to a transient receptor potential (TRP) ion channel through a G protein signaling pathway and produces a physiologic light response via membrane depolarization and increased intracellular calcium. The protein functions as a sensory photopigment and may also have photoisomerase activity. Experiments with knockout mice indicate that this gene attenuates, but does not abolish, photoentrainment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

melanopsin: Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all- trans retinal back to 11-cis retinal. Belongs to the G-protein coupled receptor 1 family. Opsin subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: integral to membrane; plasma membrane

Molecular Function: 11-cis retinal binding; G-protein coupled photoreceptor activity

Biological Process: rhodopsin mediated signaling; visual perception; rhythmic process; phototransduction; regulation of circadian rhythm; protein-chromophore linkage