Protease 7 (ompT) Recombinant Protein | ompT recombinant protein

Recombinant Escherichia coli Protease 7 (ompT)

Cat. Num. AAA1147844

• Gene Names: ompT; ECK0557; JW0554
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protease 7 (ompT); Recombinant Escherichia coli Protease 7 (ompT); Omptin; Outer membrane protein 3B; Protease A; Protease VII; ompT recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 21-317aa; Full Length of Mature Protein
• Sequence: STETLSFTPDNINADISLGTLSGKTKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYSFTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTFKYSGWVESSDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRVTNKKGNTSLYDHNNNTSDYSKNGAGIENYNFITTAGLKYTF

• Production Note: Special Offer: The E Coli host-expressed protein is manufactured from a stock plasmid containing the protein gene. E Colihost-expressed protein is stocked in different unit sizes ranging from as small as 10 ug to as large as 1 mg. Bulk inventory is also available. The E Coli host-expressed protein has been ordered over and over again by researchers and has stood the test of time as both a robust protein and important target for the research community. It is part of our new program to make our most popular protein targets and corresponding hosts available in expanded unit sizes and with a quick processing time. Select E Coli host-expressed protein for the fastest delivery among all hosts. Please contact our technical support team or email to [email protected] for more details.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-Page

Related Product Information for ompT recombinant protein

Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.

References

Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.Grodberg J., Lundrigan M.D., Toledo D.L., Mangel W.F., Dunn J.J.Nucleic Acids Res. 16:1209-1209(1988) Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues identity of protease VII and OmpT.Sugimura K., Nisihihara T.J. Bacteriol. 170:5625-5632(1988) Sequence of minutes 4-25 of Escherichia coli.Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W. The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.DNA Res. 3:137-155(1996) Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis.Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.-C., Hochstrasser D.F., Williams K.L., Gooley A.A.Electrophoresis 19:837-844(1998) Identification of OmpT as the protease that hydrolyzes the antimicrobial peptide protamine before it enters growing cells of Escherichia coli.Stumpe S., Schmid R., Stephens D.L., Georgiou G., Bakker E.P.J. Bacteriol. 180:4002-4006(1998) In vitro folding, purification and characterization of Escherichia coli outer membrane protease ompT.Kramer R.A., Zandwijken D., Egmond M.R., Dekker N.Eur. J. Biochem. 267:885-893(2000) Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site.Kramer R.A., Vandeputte-Rutten L., de Roon G.J., Gros P., Dekker N., Egmond M.R.FEBS Lett. 505:426-430(2001) Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site.Vandeputte-Rutten L., Kramer R.A., Kroon J., Dekker N., Egmond M.R., Gros P.EMBO J. 20:5033-5039(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 16128548
• NCBI GeneID: 945185
• NCBI Accession #: NP_415097.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P09169
• Molecular Weight: 37.5 kDa
• NCBI Official Full Name: DLP12 prophage; outer membrane protease VII; outer membrane protein 3b
• NCBI Official Symbol: ompT
• NCBI Official Synonym Symbols: ECK0557; JW0554
• NCBI Protein Information: DLP12 prophage; outer membrane protease VII; outer membrane protein 3b
• UniProt Protein Name: Protease 7
• Protein Family: Protease
• UniProt Gene Name: ompT
• UniProt Entry Name: OMPT_ECOLI

NCBI Description

Mg(2+) stimulon-PhoPQ regulon, EvgAS-PhoPQ cascade enhanced-expression subset. [More information is available at EcoGene: EG10673]. OmpT is an outer membrane protease with specificity for paired basic residues ; detailed studies on substrate specificity have been performed . [More information is available at EcoCyc: EG10673].

Uniprot Description

Protease that can cleave T7 RNA polymerase, ferric enterobactin receptor protein (FEP), antimicrobial peptide protamine and other proteins. This protease has a specificity for paired basic residues.