Sheep Oncoprotein Induced Transcript 3 ELISA Kit | OIT3 elisa kit

Sheep Oncoprotein Induced Transcript 3 ELISA Kit

Cat. Num. AAA738700

• Gene Names: OIT3; LZP
• Reactivity: Sheep
• Synonyms: Oncoprotein Induced Transcript 3; Sheep Oncoprotein Induced Transcript 3 ELISA Kit; OIT3 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This OIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep OIT3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OIT3 elisa kit

Principle of the assay: OIT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OIT3 antibody and an OIT3-HRP conjugate. The assay sample and buffer are incubated together with OIT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OIT3 concentration since OIT3 from samples and OIT3-HRP conjugate compete for the anti-OIT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by OIT3 from the sample, fewer sites are left to bind OIT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OIT3 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22749297
• NCBI GeneID: 170392
• NCBI Accession #: NP_689848.1
• NCBI GenBank Nucleotide #: NM_152635.1
• Molecular Weight: 34,911 Da
• NCBI Official Full Name: oncoprotein-induced transcript 3 protein
• NCBI Official Synonym Full Names: oncoprotein induced transcript 3
• NCBI Official Symbol: OIT3
• NCBI Official Synonym Symbols: LZP
• NCBI Protein Information: oncoprotein-induced transcript 3 protein; liver-specific ZP domain-containing protein; liver-specific zona pellucida domain-containing protein
• UniProt Protein Name: Oncoprotein-induced transcript 3 protein
• Protein Family: Oncoprotein-induced transcript 3 protein
• UniProt Gene Name: OIT3
• UniProt Synonym Gene Names: LZP; UNQ826/PRO1753
• UniProt Entry Name: OIT3_HUMAN

Uniprot Description

LZP: a liver-specific protein possibly involved in hepatocellular function and development. Contains 3 epidermal growth factor (EGF)-like domains and a ZP domain. Human LZP is expressed specifically in liver out of 23 tissues examined, and its mouse counterpart was detected at very early stage during embryo development.

Protein type: Secreted; Secreted, signal peptide; Calcium-binding

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: nuclear envelope

Molecular Function: calcium ion binding