U8 snoRNA-decapping enzyme (Nudt16) Recombinant Protein | Nudt16 recombinant protein

Recombinant Mouse U8 snoRNA-decapping enzyme (Nudt16)

Cat. Num. AAA1286349

• Gene Names: Nudt16; AI851783; 2310041H06Rik; 2810047L04Rik; 2900006H04Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: U8 snoRNA-decapping enzyme (Nudt16); Recombinant Mouse U8 snoRNA-decapping enzyme (Nudt16); Nudt16 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-195, full length protein
• Sequence: MEGHRKVELSEALALGPDWRHACHALLYAPDPRKLFGRIPMRFAVLMQMRFDGRLGFPGGFVDAQDSCLEDGLNRELREELGEAMSAFRVERSDYRSSHIAARPRVVAHFYAKRLTLEQLQAVEARAPQAKDHGLEVLGLVRVPLYVLRDGEGGLPAFLENSFIGAAREQLLEALQDLKLLDPGIIAKLKIPDSK
• Sequence Length: 195

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 125656157
• NCBI GeneID: 75686
• NCBI Accession #: NP_083661.2
• NCBI GenBank Nucleotide #: NM_029385.2
• UniProt Accession #: Q6P3D0; Q9D716
• Molecular Weight: 21,825 Da
• NCBI Official Full Name: U8 snoRNA-decapping enzyme
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 16
• NCBI Official Symbol: Nudt16
• NCBI Official Synonym Symbols: AI851783; 2310041H06Rik; 2810047L04Rik; 2900006H04Rik
• NCBI Protein Information: U8 snoRNA-decapping enzyme
• UniProt Protein Name: U8 snoRNA-decapping enzyme
• Protein Family: U8 snoRNA-decapping enzyme
• UniProt Gene Name: Nudt16
• UniProt Synonym Gene Names: IDPase; Nudix motif 16

Uniprot Description

RNA-binding and decapping enzyme that catalyzes the cleavage of the cap structure of snoRNAs and mRNAs in a metal-dependent manner. Part of the U8 snoRNP complex that is required for the accumulation of mature 5.8S and 28S rRNA. Has diphosphatase activity and removes m7G and/or m227G caps from U8 snoRNA and leaves a 5'monophosphate on the RNA. Catalyzes also the cleavage of the cap structure on mRNAs. Does not hydrolyze cap analog structures like 7-methylguanosine nucleoside triphosphate (m7GpppG). Also hydrolysis m7G- and m227G U3-capped RNAs but with less efficiencies. Has broad substrate specificity with manganese or cobalt as cofactor and can act on various RNA species. Binds to the U8 snoRNA; metal is not required for RNA-binding. May play a role in the regulation of snoRNAs and mRNAs degradation (). Acts also as a phosphatase; hydrolyzes the non-canonical purine nucleotides inosine diphosphate (IDP) and deoxyinosine diphosphate (dITP) as well as guanosine diphosphate (GDP), deoxyguanosine diphosphate (dGDP), xanthine diphosphate (XDP), inosine triphosphate (ITP) and deoxyinosine triphosphate (ITP) to their respective monophosphate derivatives and does not distinguish between the deoxy- and ribose forms. The order of activity with different substrates is IDP > dIDP >> GDP = dGDP > XDP = ITP = dITP. Binds strongly to GTP, ITP and XTP. Participates in the hydrolysis of dIDP/IDP and probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.