Human 7,8-dihydro-8-oxoguanine triphosphatase ELISA Kit | NUDT1 elisa kit

Human 7,8-dihydro-8-oxoguanine triphosphatase ELISA Kit

Cat. Num. AAA2887109

• Gene Names: NUDT1; MTH1
• Reactivity: Human
• Synonyms: 7; 8-dihydro-8-oxoguanine triphosphatase; Human 7; 8-dihydro-8-oxoguanine triphosphatase ELISA Kit; 8-oxo-dGTPase; Nucleoside diphosphate-linked moiety X motif 1; Nudix motif 1; NUDT1; MTH1; 3.6.1.55; 3.6.1.56; Nucleoside diphosphate-linked moiety; NUDT1 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human 7,8-dihydro-8-oxoguanine triphosphatase
• Sequence Length: 156

• Detection Range: 0.78-50 ng/mL
• Intra-assay Precision: Intra-Assay CV: ?4.2%
• Inter-assay Precision: Inter-Assay CV: ?8.1%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 40288274
• NCBI GeneID: 4521
• NCBI Accession #: NP_002443.3
• NCBI GenBank Nucleotide #: NM_002452.3
• UniProt Accession #: P36639; Q6LES7; Q6P0Y6; Q7Z7N6; Q8IV95; Q9UBM0; Q9UBM9; A4D205
• Molecular Weight: 17,952 Da
• NCBI Official Full Name: 7,8-dihydro-8-oxoguanine triphosphatase isoform p18
• NCBI Official Synonym Full Names: nudix hydrolase 1
• NCBI Official Symbol: NUDT1
• NCBI Official Synonym Symbols: MTH1
• NCBI Protein Information: 7,8-dihydro-8-oxoguanine triphosphatase
• UniProt Protein Name: 7,8-dihydro-8-oxoguanine triphosphatase
• Protein Family: 7,8-dihydro-8-oxoguanine triphosphatase
• UniProt Gene Name: NUDT1
• UniProt Synonym Gene Names: MTH1; Nudix motif 1
• UniProt Entry Name: 8ODP_HUMAN

NCBI Description

Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. [provided by RefSeq, Jul 2008]

Uniprot Description

NUDT1: Antimutagenic. Acts as a sanitizing enzyme for oxidized nucleotide pools, thus suppressing cell dysfunction and death induced by oxidative stress. Hydrolyzes 8-oxo-dGTP, 8-oxo-dATP and 2-OH-dATP, thus preventing misincorporation of oxidized purine nucleoside triphosphates into DNA and subsequently preventing A:T to C:G and G:C to T:A transversions. Able to hydrolyze also the corresponding ribonucleotides, 2-OH-ATP, 8-oxo-GTP and 8-oxo-ATP. Belongs to the Nudix hydrolase family. 4 isoforms of the human protein are produced by alternative initiation.

Protein type: Hydrolase; EC 3.6.1.55; EC 3.6.1.56

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cytoplasm; cytosol; mitochondrial matrix; mitochondrion; nucleus

Molecular Function: 8-oxo-7,8-dihydroguanine triphosphatase activity; ATP diphosphatase activity; GTPase activity; m7G(5')pppN diphosphatase activity; metal ion binding; protein binding; snoRNA binding

Biological Process: dATP catabolic process; dGTP catabolic process; DNA protection; DNA repair; nucleobase, nucleoside and nucleotide metabolic process; purine nucleotide catabolic process; response to oxidative stress