Sequence
Nucleobindin 1 Recombinant Protein | NUCB1 recombinant protein
Recombinant Nucleobindin 1 (NUCB1)
Gene Names
NUCB1; NUC; CALNUC
Reactivity
Homo sapiens (Human)
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Nucleobindin 1; Recombinant Nucleobindin 1 (NUCB1); NUC; CALNUC; NUCB1 recombinant protein
Host
E.coli
Reactivity
Homo sapiens (Human)
Purity/Purification
> 90%
Form/Format
100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 1000ug/mL (varies by lot)
Applicable Applications for NUCB1 recombinant protein
Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Glu37~Leu461
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
5.1
Usage
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted Molecular Mass: 53.8kDa
Accurate Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 54kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
Nucleobindin 1
NCBI Official Synonym Full Names
nucleobindin 1
NCBI Official Symbol
NUCB1
NCBI Official Synonym Symbols
NUC; CALNUC
NCBI Protein Information
nucleobindin-1
UniProt Protein Name
Nucleobindin-1
Protein Family
UniProt Gene Name
NUCB1
UniProt Synonym Gene Names
NUC
UniProt Entry Name
NUCB1_HUMAN
NCBI Description
This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]
Uniprot Description
NUCB1: Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis. Belongs to the nucleobindin family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: cis-Golgi network; early endosome; ER-Golgi intermediate compartment; extracellular space; Golgi apparatus; Golgi-associated vesicle; membrane; microtubule cytoskeleton; nucleus; rough endoplasmic reticulum; trans-Golgi network
Molecular Function: calcium ion binding; DNA binding; G-protein alpha-subunit binding; protein binding
Research Articles on NUCB1
Similar Products
Product Notes
The NUCB1 nucb1 (Catalog #AAA2034499) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Nucleobindin 1 (NUCB1) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleobindin 1 can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the NUCB1 nucb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nucleobindin 1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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