Endonuclease-III Recombinant Protein | NTH recombinant protein
Recombinant E Coli Endonuclease-III
Gene Names
nth; ECK1629; JW1625
Purity
Greater than 85.0% as determined by SDS-PAGE.
Synonyms
Endonuclease-III; Recombinant E Coli Endonuclease-III; NTH E.Coli; Endonuclease-III E.Coli Recombinant; DNA-(apurinic or apyrimidinic site) lyase; b1633; JW1625; NTH recombinant protein
Host
E Coli
Purity/Purification
Greater than 85.0% as determined by SDS-PAGE.
Form/Format
The NTH solution (0.5mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT, 0.1mM PMSF and 40% glycerol.
Sterile Filtered colorless solution.
Sterile Filtered colorless solution.
Sequence
MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I
Sequence Length
211
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Related Product Information for NTH recombinant protein
Description: NTH E Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211a.a.) and having a molecular mass of 25.7kDa. The NTH is purified by proprietary chromatographic techniques.
Introduction: Endonuclease III (nth) is a DNA repair enzyme which has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases numerous damaged pyrimidines from DNA by cleaving the N-glycosidic bond and leaving an AP (apurinic/apyrimidinic) site. This AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, thus leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Introduction: Endonuclease III (nth) is a DNA repair enzyme which has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases numerous damaged pyrimidines from DNA by cleaving the N-glycosidic bond and leaving an AP (apurinic/apyrimidinic) site. This AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, thus leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Product Categories/Family for NTH recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,562 Da
NCBI Official Full Name
DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
NCBI Official Symbol
nth
NCBI Official Synonym Symbols
ECK1629; JW1625
NCBI Protein Information
DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
UniProt Protein Name
Endonuclease III
Protein Family
UniProt Gene Name
nth
UniProt Entry Name
END3_ECOLI
NCBI Description
Nth is specific for damaged pyrimidine sites, particularly Thy. [More information is available at EcoGene: EG10662]. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction. [More information is available at EcoCyc: EG10662].
Uniprot Description
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Research Articles on NTH
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Product Notes
The NTH nth (Catalog #AAA144942) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MGSSHHH HHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I. It is sometimes possible for the material contained within the vial of "Endonuclease-III, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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