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RNA1 polyprotein Recombinant Protein | CPMVs1gp1 recombinant protein

Recombinant Cowpea mosaic virus RNA1 polyprotein

Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
RNA1 polyprotein; Recombinant Cowpea mosaic virus RNA1 polyprotein; Recombinant RNA1 polyprotein; Genome polyprotein B P1 Cleaved into the following 5 chains: 1. Protease cofactor; 32 kDa protein Putative helicase EC= 3.6.4.-; 58 kDa protein Membrane-binding protein NTP-binding protein; NTB Viral genome-; CPMVs1gp1 recombinant protein
Ordering
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1156-1866
Sequence
GAEEYFDFLPAEENVSSGVAMVAGLKQGVYIPLPTKTALVETPSEWHLDTPCDKVPSILVPTDPRIPAQHEGYDPAKSGVSKYSQPMSALDPELLGEVANDVLELWHDCAVDWDDFGEVSLEEALNGCEGVEYMERIPLATSEGFPHILSRNGKEKGKRRFVQGDDCVVSLIPGTTVAKAYEELEASAHRFVPALVGIECPKDEKLPMRKVFDKPKTRCFTILPMEYNLVVRRKFLNFVRFIMANRHRLSCQVGINPYSMEWSRLAARMKEKGNDVLCCDYSSFDGLLSKQVMDVIASMINELCGGEDQLKNARRNLLMACCSRLAICKNTVWRVECGIPSGFPMTVIVNSIFNEILIRYHYKKLMREQQAPELMVQSFDKLIGLVTYGDDNLISVNAVVTPYFDGKKLKQSLAQGGVTITDGKDKTSLELPFRRLEECDFLKRTFVQRSSTIWDAPEDKASLWSQLHYVNCNNCEKEVAYLTNVVNVLRELYMHSPREATEFRRKVLKKVSWITSGDLPTLAQLQEFYEYQRQQGGADNNDTCDLLTSVDLLGPPLSFEKEAMHGCKVSEEIVTKNLAYYDFKRKGEDEVVFLFNTLYPQSSLPDGCHSVTWSQGSGRGGLPTQSWMSYNISRKDSNINKIIRTAVSSKKRVIFCARDNMVPVNIVALLCAVRNKLMPTAVSNATLVKVMENAKAFKFLPEEFNFAFSDV
Sequence Length
1866
Species
Cowpea mosaic virus (strain SB) (CPMV)
Preparation and Storage
Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
209,810 Da
NCBI Official Full Name
polyprotein
NCBI Official Symbol
CPMVs1gp1
NCBI Protein Information
polyprotein; protease cofactor; membrane-binding protein; vpg; RNA-dependent RNA polymerase
UniProt Protein Name
RNA1 polyprotein
Protein Family
UniProt Gene Name
NTB
UniProt Synonym Gene Names
3C-like protease
UniProt Entry Name
POL1_CPMVS

Uniprot Description

Function: Picornain 3C-like protease is a thiol protease that probably cleaves the B and M polyproteins. Ref.3VPg plays a role in RNA replication. Ref.3

Catalytic activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Subcellular location: Putative helicase: Host membrane; Single-pass membrane protein

Potential.

Post-translational modification: Specific enzymatic cleavages by picornain 3C-like protease in vivo yield mature proteins. Picornain 3C-like protease is autocatalytically processed

By similarity.VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase.

Sequence similarities: Belongs to the comoviridae genome polyprotein B family.Contains 1 peptidase C3 domain.Contains 1 RdRp catalytic domain.Contains 1 SF3 helicase domain.

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Product Notes

The CPMVs1gp1 ntb (Catalog #AAA1042980) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1156-1866. The amino acid sequence is listed below: GAEEYFDFLP AEENVSSGVA MVAGLKQGVY IPLPTKTALV ETPSEWHLDT PCDKVPSILV PTDPRIPAQH EGYDPAKSGV SKYSQPMSAL DPELLGEVAN DVLELWHDCA VDWDDFGEVS LEEALNGCEG VEYMERIPLA TSEGFPHILS RNGKEKGKRR FVQGDDCVVS LIPGTTVAKA YEELEASAHR FVPALVGIEC PKDEKLPMRK VFDKPKTRCF TILPMEYNLV VRRKFLNFVR FIMANRHRLS CQVGINPYSM EWSRLAARMK EKGNDVLCCD YSSFDGLLSK QVMDVIASMI NELCGGEDQL KNARRNLLMA CCSRLAICKN TVWRVECGIP SGFPMTVIVN SIFNEILIRY HYKKLMREQQ APELMVQSFD KLIGLVTYGD DNLISVNAVV TPYFDGKKLK QSLAQGGVTI TDGKDKTSLE LPFRRLEECD FLKRTFVQRS STIWDAPEDK ASLWSQLHYV NCNNCEKEVA YLTNVVNVLR ELYMHSPREA TEFRRKVLKK VSWITSGDLP TLAQLQEFYE YQRQQGGADN NDTCDLLTSV DLLGPPLSFE KEAMHGCKVS EEIVTKNLAY YDFKRKGEDE VVFLFNTLYP QSSLPDGCHS VTWSQGSGRG GLPTQSWMSY NISRKDSNIN KIIRTAVSSK KRVIFCARDN MVPVNIVALL CAVRNKLMPT AVSNATLVKV MENAKAFKFL PEEFNFAFSD V. It is sometimes possible for the material contained within the vial of "RNA1 polyprotein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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