Human Neurotrophin 3 ELISA Kit | NT 3 elisa kit

Human Neurotrophin 3 ELISA Kit

Cat. Num. AAA728456

• Gene Names: NTF3; NT3; HDNF; NGF2; NT-3; NGF-2
• Reactivity: Human
• Synonyms: Neurotrophin 3; Human Neurotrophin 3 ELISA Kit; NT 3 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This NT-3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NT-3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NT 3 elisa kit

Principle of the Assay: NT-3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NT-3 antibody and an NT-3-HRP conjugate. The assay sample and buffer are incubated together with NT-3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NT-3 concentration since NT-3 from samples and NT-3-HRP conjugate compete for the anti-NT-3 antibody binding site. Since the number of sites is limited, as more sites are occupied by NT-3 from the sample, fewer sites are left to bind NT-3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NT-3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NT 3 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 4505469
• NCBI GeneID: 4908
• NCBI Accession #: NP_002518.1
• NCBI GenBank Nucleotide #: NM_002527.4
• Molecular Weight: 30,800 Da
• NCBI Official Full Name: neurotrophin-3 isoform 2 preproprotein
• NCBI Official Synonym Full Names: neurotrophin 3
• NCBI Official Symbol: NTF3
• NCBI Official Synonym Symbols: NT3; HDNF; NGF2; NT-3; NGF-2
• NCBI Protein Information: neurotrophin-3; neurotrophic factor; nerve growth factor 2
• UniProt Protein Name: Neurotrophin-3
• UniProt Gene Name: NTF3
• UniProt Synonym Gene Names: NT-3; NGF-2
• UniProt Entry Name: NTF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]

Uniprot Description

NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region

Molecular Function: neurotrophin p75 receptor binding; growth factor activity; nerve growth factor binding; receptor binding; chemoattractant activity

Biological Process: myelination; axon guidance; epidermis development; mechanoreceptor differentiation; glial cell fate determination; regulation of neuron differentiation; activation of MAPK activity; positive regulation of receptor internalization; positive regulation of glial cell differentiation; signal transduction; enteric nervous system development; induction of positive chemotaxis; cell-cell signaling; positive chemotaxis; positive regulation of cell proliferation; negative regulation of neuron apoptosis; regulation of synaptic transmission; negative regulation of peptidyl-tyrosine phosphorylation; nervous system development; activation of protein kinase B; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; neuromuscular synaptic transmission; nerve development; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; brain development; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis; positive regulation of cell migration