NSUN2 recombinant protein

Recombinant Human NSUN2 protein

Cat. Num. AAA1561415

• Gene Names: NSUN2; MISU; MRT5; SAKI; TRM4
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NSUN2; Recombinant Human NSUN2 protein; RNA cytosine C(5)-methyltransferase NSUN2; Myc-induced SUN domain-containing protein; Misu; NOL1/NOP2/Sun domain family member 2; Substrate of AIM1/Aurora kinase B; mRNA cytosine C(5)-methyltransferase; tRNA cytosine C(5)-methyltransferase; tRNA methyltransferase 4 homolog; hTrm4; NSUN2 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human NSUN2 (His415-Arg767) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for NSUN2 recombinant protein

RNA cytosine C5-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C5-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C5-methylation of mitochondrial tRNAs. Catalyzes cytosine C5-methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts. Cytosine C5-methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling. Also mediates cytosine C5-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs. Cytosine C5-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.

Product Categories/Family for NSUN2 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 148887180
• NCBI GeneID: 54888
• UniProt Accession #: Q08J23; Q9BVN4; Q9H858; Q9NXD9; A8K529; B2RNR4; B3KP09; B4DQW2; G3V1R4
• Molecular Weight: 767
• NCBI Official Full Name: tRNA (cytosine(34)-C(5))-methyltransferase
• NCBI Official Synonym Full Names: NOP2/Sun RNA methyltransferase family, member 2
• NCBI Official Symbol: NSUN2
• NCBI Official Synonym Symbols: MISU; MRT5; SAKI; TRM4
• NCBI Protein Information: tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine(34)-C(5))-methyltransferase; NOP2/Sun domain family, member 2; tRNA methyltransferase 4 homolog; substrate of AIM1/Aurora kinase B; 5-methycytoisine methyltransferase; NOL1/NOP2/Sun domain family,
• UniProt Protein Name: tRNA (cytosine(34)-C(5))-methyltransferase
• Protein Family: tRNA
• UniProt Gene Name: NSUN2
• UniProt Synonym Gene Names: SAKI; TRM4; Misu; hTrm4
• UniProt Entry Name: NSUN2_HUMAN

NCBI Description

This gene encodes a methyltransferase that catalyzes the methylation of cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. This modification is necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Mar 2011]

Uniprot Description

NSUN2: an RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. Interacts with NPM1 and NCL during interphase. Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA. Defects in NSUN2 are the cause of mental retardation autosomal recessive type 5 (MRT5), a disorder characterized by significantly below average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. Two human isoforms produced by alternative splicing.

Protein type: EC 2.1.1.203; Methyltransferase; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 5p15.31

Cellular Component: cytoplasm; nucleolus; spindle; nucleus

Molecular Function: tRNA (cytosine-5-)-methyltransferase activity; tRNA binding

Biological Process: mitosis; cell division; tRNA methylation

Disease: Mental Retardation, Autosomal Recessive 5