SFRP2 active protein

SFRP2, Recombinant, Mouse (Secreted Frizzled Related Protein 2, SARP-1, SDF-5) (BSA Free)

Cat. Num. AAA635749

• Gene Names: SFRP2; FRP-2; SARP1; SDF-5
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
• Synonyms: SFRP2; Recombinant; Mouse (Secreted Frizzled Related Protein 2; SARP-1; SDF-5) (BSA Free); SFRP2 active protein

• Host: NSO (Recombinant, Mouse)
• Specificity: Species sequence homology: Human -98%
• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile PBS to 0.1mg/ml.

• Activity: Measured by its ability to compete for the binding of rmFrizzled-1 and biotinylated rmWnt-3a. rmsFRP-2 has an IC 50 value of 0.7-3.5nM, under conditions in which rmFrizzled-1/Fc is present at 5.4nM, and biotinylated rmWnt-3a concentration is 2.7nM.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for SFRP2 active protein

Secreted Frizzled Related Proteins (sFRPs) are a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. The sFRPs are approximately 30-35kD in size and are comprised of 3 domains: a signal sequence; a cysteine-rich domain (CRD) of about 110 amino acids (aa) with high degree of similarity to the

Recombinant protein corresponding to Leu25-Cys295 from mouse SFRP2 with a C-terminal 6-His tag expressed in NSO cells (AAB70795).

Product Categories/Family for SFRP2 active protein

Molecular Biology; MB-Wnt Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 48475052
• NCBI GeneID: 6423
• NCBI Accession #: NP_003004.1
• NCBI GenBank Nucleotide #: NM_003013.2
• UniProt Accession #: Q96HF1; O14778; Q9HAP5; B3KQR2
• Molecular Weight: ~31.6kD
• NCBI Official Full Name: secreted frizzled-related protein 2
• NCBI Official Synonym Full Names: secreted frizzled-related protein 2
• NCBI Official Symbol: SFRP2
• NCBI Official Synonym Symbols: FRP-2; SARP1; SDF-5
• NCBI Protein Information: secreted frizzled-related protein 2; SARP-1; sFRP-2; secreted apoptosis related protein 1; secreted apoptosis-related protein 1
• UniProt Protein Name: Secreted frizzled-related protein 2
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP2
• UniProt Synonym Gene Names: FRP2; SARP1; FRP-2; sFRP-2; SARP-1
• UniProt Entry Name: SFRP2_HUMAN

NCBI Description

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: extracellular matrix; extracellular space; integral to membrane

Molecular Function: integrin binding; G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; fibronectin binding; receptor agonist activity

Biological Process: convergent extension involved in axis elongation; collagen fibril organization; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; negative regulation of caspase activity; negative regulation of BMP signaling pathway; negative regulation of cell proliferation; negative regulation of mesodermal cell fate specification; cell-cell signaling; positive regulation of cell proliferation; negative regulation of cell migration; response to nutrient; negative regulation of epithelial cell proliferation; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of JNK activity; male gonad development; positive regulation of cell adhesion mediated by integrin; digestive tract morphogenesis; chondrocyte development; positive regulation of cell growth; positive regulation of peptidyl-serine phosphorylation; patterning of blood vessels; positive regulation of osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; cellular response to extracellular stimulus; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent