Genome polyprotein Recombinant Protein | NS1 recombinant protein
Recombinant Dengue virus type 2 Genome polyprotein
NCBI and Uniprot Product Information
Uniprot Description
Function: Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes
By similarity.Non-structural protein 1 is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome
By similarity.Non-structural protein 2A may be involved viral RNA replication and capsid assembly
Potential.Non-structural protein 2B is a required cofactor for the serine protease function of NS3
By similarity.Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction
By similarity.
Catalytic activity: Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.NTP + H2O = NDP + phosphate.ATP + H2O = ADP + phosphate.
Subunit structure: In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E. NS1 forms homodimers as well as homohexamers when secreted. NS1 may interact with NS4A. NS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter, acting as a cofactor. In the absence of the NS2B, NS3 protease is unfolded and inactive
By similarity.
Subcellular location: Envelope protein E: Virion membrane; Multi-pass membrane protein
By similarity. Host endoplasmic reticulum membrane; Multi-pass membrane protein
By similarity. Non-structural protein 1: Secreted. Host endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side
By similarity. Non-structural protein 2A-alpha: Host endoplasmic reticulum membrane; Multi-pass membrane protein
Potential. Non-structural protein 2A: Host endoplasmic reticulum membrane; Multi-pass membrane protein
Potential. Serine protease NS3: Host endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side
By similarity. Note: Remains non-covalently associated to NS3 protease
By similarity.
Post-translational modification: Specific enzymatic cleavages in vivo yield mature proteins
By similarity.
Sequence similarities: Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.Contains 1 peptidase S7 domain.
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Product Notes
The Genome polyprotein ns1 (Catalog #AAA1161902) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1066-1683. The amino acid sequence is listed below: AGVLWDVPSP PPVGKAELED GAYRIKQKGI LGYSQIGAGV YKEGTFHTMW HVTRGAVLMH KGKRIEPSWA DVKKDLISYG GGWKLEGEWK EGEEVQVLAL EPGKNPRAVQ TKPGLFRTNT GTIGAVSLDF SPGTSGSPIV DKKGKVVGLY GNGVVTRSGA YVSAIAQTEK SIEDNPEIED DIFRKRRLTI MDLHPGAGKT KRYLPAIVRE AIKRGLRTLI LAPTRVVAAE MEEALRGLPI RYQTPAIRAE HTGREIVDLM CHATFTMRLL SPIRVPNYNL IIMDEAHFTD PASIAARGYI STRVEMGEAA GIFMTATPPG SRDPFPQSNA PIMDEEREIP ERSWNSGHEW VTDFKGKTVW FVPSIKTGND IAACLRKNGK RVIQLSRKTF DSEYVKTRTN DWDFVVTTDI SEMGANFKAE RVIDPRRCMK PVILTDGEER VILAGPMPVT HSSAAQRRGR IGRNPRNEND QYIYMGEPLE NDEDCAHWKE AKMLLDNINT PEGIIPSIFE PEREKVDAID GEYRLRGEAR KTFVDLMRRG DLPVWLAYKV AAEGINYADR RWCFDGTRNN QILEENVEVE IWTKEGERKK LKPRWLDARI YSDPLALKEF KEFAAGRK. It is sometimes possible for the material contained within the vial of "Genome polyprotein, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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