DNA-directed RNA polymerase II subunit RPB3-A (RPB36A) Recombinant Protein | RPB36A recombinant protein

Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB3-A (RPB36A)

Cat. Num. AAA1419683

• Gene Names: NRPB3; F26H6.5; F26H6_5; NRPD3; NRPE3A; RBP36A; RPB35.5A
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: DNA-directed RNA polymerase II subunit RPB3-A (RPB36A); Recombinant Arabidopsis thaliana DNA-directed RNA polymerase II subunit RPB3-A (RPB36A); RPB36A recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-319, Full length protein
• Sequence: MDGATYQRFPKIKIRELKDDYAKFELRETDVSMANALRRVMISEVPTVAIDLVEIEVNSSVLNDEFIAHRLGLIPLTSERAMSMRFSRDCDACDGDGQCEFCSVEFRLSSKCVTDQTLDVTSRDLYSADPTVTPVDFTIDSSVSDSSEHKGIIIVKLRRGQELKLRAIARKGIGKDHAKWSPAATVTFMYEPDIIINEDMMDTLSDEEKIDLIESSPTKVFGMDPVTRQVVVVDPEAYTYDEEVIKKAEAMGKPGLIEISPKDDSFIFTVESTGAVKASQLVLNAIDLLKQKLDAVRLSDDTVEADDQFGELGAHMRGG
• Sequence Length: 319

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 15226523
• NCBI GeneID: 816035
• NCBI Accession #: NP_179145.1
• NCBI GenBank Nucleotide #: NM_127103.3
• UniProt Accession #: Q39211
• Molecular Weight: 35,461 Da
• NCBI Official Full Name: DNA-directed RNA polymerase family protein
• NCBI Official Symbol: NRPB3
• NCBI Official Synonym Symbols: F26H6.5; F26H6_5; NRPD3; NRPE3A; RBP36A; RPB35.5A
• NCBI Protein Information: DNA-directed RNA polymerase family protein
• UniProt Protein Name: DNA-directed RNA polymerases II, IV and V subunit 3
• UniProt Gene Name: NRPB3
• UniProt Synonym Gene Names: NRPD3; NRPE3A; RPB36A; RNA polymerase II subunit 3-A; RNA polymerase II subunit B3-A

NCBI Description

Non-catalytic subunit of nuclear DNA-dependent RNA polymerases II, IV and V; homologous to budding yeast RPB3 and the E. coli RNA polymerase alpha subunit. A closely related paralog, encoded by At2g15400, can substitute for At2g15430 in the context of Pol V.

Uniprot Description

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. NRPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft. Component of RNA polymerases IV and V which mediate short-interfering RNAs (siRNA) accumulation and subsequent RNA-directed DNA methylation-dependent (RdDM) transcriptional gene silencing (TGS) of endogenous repeated sequences, including transposable elements.