NAD (P)H dehydrogenase [quinone] 1 (Nqo1) Recombinant Protein | Nqo1 recombinant protein

Recombinant Mouse NAD (P)H dehydrogenase [quinone] 1 (Nqo1)

Cat. Num. AAA9423826

• Gene Names: Nqo1; Dtd; Ox1; Qr1; Dia4; Nmo1; Ox-1; Nmo-1; Nmor1; AV001255
• Reactivity: Mouse
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: NAD (P)H dehydrogenase [quinone] 1 (Nqo1); Recombinant Mouse NAD (P)H dehydrogenase [quinone] 1 (Nqo1); Azoreductase; Nqo1 recombinant protein

• Host: Yeast
• Reactivity: Mouse
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Tris-based buffer, 50% glycerol
• Sequence Positions: Full Length, 2-274aa

• Tag Info: N-terminal 6xHis-tagged
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.

Related Product Information for Nqo1 recombinant protein

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.

NCBI and Uniprot Product Information

• NCBI GI #: 94400773
• NCBI GeneID: 18104
• NCBI Accession #: NP_032732.3
• NCBI GenBank Nucleotide #: NM_008706.5
• UniProt Accession #: Q64669
• Molecular Weight: 32.83 kDa
• NCBI Official Full Name: NAD(P)H dehydrogenase
• NCBI Official Synonym Full Names: NAD(P)H dehydrogenase, quinone 1
• NCBI Official Symbol: Nqo1
• NCBI Official Synonym Symbols: Dtd; Ox1; Qr1; Dia4; Nmo1; Ox-1; Nmo-1; Nmor1; AV001255
• NCBI Protein Information: NAD(P)H dehydrogenase [quinone] 1
• UniProt Protein Name: NAD(P)H dehydrogenase [quinone] 1
• Protein Family: NAD(P)H dehydrogenase
• UniProt Gene Name: Nqo1
• UniProt Synonym Gene Names: Dia4; Nmo1; Nmor1; DTD; QR1

Uniprot Description

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer's disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.6.5.2; Oxidoreductase

Chromosomal Location of Human Ortholog: 8 D3|8 54.08 cM

Cellular Component: cell soma; cytosol

Molecular Function: identical protein binding; NAD(P)H dehydrogenase (quinone) activity; RNA binding; superoxide dismutase activity

Biological Process: negative regulation of apoptosis; negative regulation of catalytic activity; positive regulation of neuron apoptosis; response to oxidative stress; superoxide metabolic process