NPPB/NT-PROBNP recombinant protein

Recombinant Human NPPB/NT-PROBNP protein, N-His Tag

Cat. Num. AAA157427

• Gene Names: NPPB; BNP
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NPPB/NT-PROBNP; Recombinant Human NPPB/NT-PROBNP protein; N-His Tag; NPPB/NT-PROBNP recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Human
• Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details
• Construction: A DNA sequence encoding the human NPPB (His27~Arg102) was fused with the N-terminal His Tag
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; Shipping: In general, proteins are provided as lyophilized powder/frozen liquid.

SDS-Page

Product Categories/Family for NPPB/NT-PROBNP recombinant protein

Other proteins; IVD material

NCBI and Uniprot Product Information

• NCBI GI #: 4505433
• NCBI GeneID: 4879
• NCBI Accession #: NP_002512.1
• NCBI GenBank Nucleotide #: NM_002521.2
• UniProt Accession #: P16860; Q6FGY0; Q9P2Q7; B0ZBE9
• Molecular Weight: 14,726 Da
• NCBI Official Full Name: natriuretic peptides B preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B; natriuretic protein; brain type natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32
• UniProt Entry Name: ANFB_HUMAN

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Jul 2008]

Uniprot Description

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Secreted; Secreted, signal peptide; Hormone

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; protein complex; extracellular region

Molecular Function: diuretic hormone activity; hormone activity; receptor binding

Biological Process: cGMP biosynthetic process; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of vasodilation; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; regulation of vascular permeability; negative regulation of cell growth; regulation of blood vessel size; body fluid secretion