NPM1-ALK recombinant protein

NPM1-ALK Mutant (F1174L), Active

Cat. Num. AAA515450

• Applications: Kinase Assay
• Synonyms: NPM1-ALK; NPM1-ALK Mutant (F1174L); Active; NPM1-ALK recombinant protein

• Host: Sf9 insect cells using baculovirus
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Positions: 1-117 (NPM1) 1058-end (F1174L) (ALK)
• Sequence Length: 2043

• Applicable Applications for NPM1-ALK recombinant protein: Kinase Assay
• Species: Human
• Tag: GST tag
• Preparation and Storage: Store product at -70 degree C.; For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.; For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Testing Data

Testing Data #2

Related Product Information for NPM1-ALK recombinant protein

Description: Recombinant human fusion protein NPM1 (1-117)-ALK (F1174L) (1058-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: NPM1-ALK which belongs to the insulin receptor superfamily which comprises an extracellular domain, a hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. CD246 plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. ALK-positive neoplasms represent a distinct entity because the morphology of the tumors is often neither anaplastic nor large cell and the tumors should be referred to as ALK lymphomas (1). CD246 has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. STAT5A is epigenetically silenced by the tyrosine kinase NPM1-ALK and acts as a tumor suppressor by reciprocally inhibiting NPM1-ALK expression (2).

Product Categories/Family for NPM1-ALK recombinant protein

Enzymes; Active Kinase Mutants

References

1. Benharroch, D.et.al: ALK-positive lymphoma: a single disease with a broad spectrum of morphology. Blood 91: 2076-2084, 1998. 2. Zhang, Q. et.al: STAT5A is epigenetically silenced by the tyrosine kinase NPM1-ALK and acts as a tumor suppressor by reciprocally inhibiting NPM1-ALK expression.

NCBI and Uniprot Product Information

• NCBI GI #: 609341
• UniProt Accession #: U04946
• Molecular Weight: ~108 kDa
• NCBI Official Full Name: Human nucleophosmin-anaplastic lymphoma kinase fusion protein (NPM/ALK) mRNA, complete cds

Uniprot Description

NPM1: a nucleolar protein associated with nucleolar ribonucleoprotein structures and that binds single-stranded nucleic acids. Is a major component of template activating factor (TAF)-III. It plays a role in controlling centrosome duplication, and may function in the assembly and/or transport of the ribosome. Two alternatively spliced isoforms have been described.

Protein type: Nucleolus; Oncoprotein; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: centrosome; cytoplasm; cytosol; focal adhesion; membrane; nucleolus; nucleoplasm; nucleus; protein complex; ribonucleoprotein complex; spindle pole centrosome

Molecular Function: histone binding; NF-kappaB binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein kinase inhibitor activity; ribosomal large subunit binding; ribosomal small subunit binding; RNA binding; Tat protein binding; transcription activator binding; transcription coactivator activity; transcription factor binding; unfolded protein binding

Biological Process: activation of NF-kappaB transcription factor; cell aging; centrosome cycle; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; DNA replication-independent nucleosome assembly at centromere; intracellular protein transport; negative regulation of apoptosis; negative regulation of cell proliferation; nucleocytoplasmic transport; nucleosome assembly; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translation; protein localization; protein oligomerization; regulation of centriole replication; regulation of endodeoxyribonuclease activity; regulation of transcription from RNA polymerase II promoter; response to stress; ribosome assembly