Nephrosis 2 Idiopathic Steroid-Resistant Recombinant Protein | NPHS2 recombinant protein
Recombinant Human Nephrosis 2 Idiopathic Steroid-Resistant
Gene Names
NPHS2; PDCN; SRN1
Purity
Purity as determined by densitometric image analysis is greater than 95%.
Synonyms
Nephrosis 2 Idiopathic Steroid-Resistant; Recombinant Human Nephrosis 2 Idiopathic Steroid-Resistant; NPHS2 Human; Nephrosis 2 Idiopathic Steroid-Resistant Human Recombinant; Nephrosis 2; Idiopathic; Steroid-Resistant (Podocin); PDCN; SRN1; Podocin; NPHS2; NPHS2 recombinant protein
Host
E Coli
Purity/Purification
Purity as determined by densitometric image analysis is greater than 95%.
Form/Format
NPHS2 was filtered (0.4 um) and lyophilized in 30mM acetate buffer and 1mM dithiothreitol, pH 4.
Filtered White lyophilized (freeze-dried) powder.
Filtered White lyophilized (freeze-dried) powder.
Sequence
MKHHHHHHASVKVVQEYERV IIFRLGHLLP GRAKGPGLFF FLPCLDTYHK VDLRLQTLEI PFHEIVTKDM FIMEIDAICY YRMENASLLL SSLAHVSKAV QFLVQTTMKR LLAHRSLTEI LLERKSIAQD AKVALDSVTC IWGIKVERIE IKDVRLPAGL QHSLAVEAEA QRQAKVRMIA AEAEKAASES LRMAAEILSG TPAAVQLRYL HTLQSLSTEK PSTVVLPLPF DLLNCLSSPS NRTQGSLPFP SPSKPVEPLN PKKKDSPML.
Sequence Length
315
Solubility
It is recommended to add 0.1M acetate buffer pH 4.0 to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited. NPHS2 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Preparation and Storage
Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.
Related Product Information for NPHS2 recombinant protein
Introduction: Nephrosis 2 Idiopathic Steroid-Resistant (NPHS2) is a protein which has a role in the regulation of glomerular permeability, serving as a linker between the plasma membrane and the cytoskeleton. NPHS2 gene mutations cause steroid-resistant nephrotic syndrome.
Description: NPHS2 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain (Val125-Leu383) containing 269 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 30.2kDa.
Description: NPHS2 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain (Val125-Leu383) containing 269 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 30.2kDa.
Product Categories/Family for NPHS2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,421 Da
NCBI Official Full Name
podocin isoform 2
NCBI Official Synonym Full Names
NPHS2 podocin
NCBI Official Symbol
NPHS2
NCBI Official Synonym Symbols
PDCN; SRN1
NCBI Protein Information
podocin
UniProt Protein Name
Podocin
Protein Family
UniProt Gene Name
NPHS2
UniProt Entry Name
PODO_HUMAN
Uniprot Description
NPHS2: Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton. Defects in NPHS2 are the cause of nephrotic syndrome type 2 (NPHS2). It is a renal disorder characterized clinically by childhood onset of proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Kidney biopsies show non-specific histologic changes such as focal segmental glomerulosclerosis and diffuse mesangial proliferation. The disorder is resistant to steroid treatment and progresses to end-stage renal failure in the first or second decades. Some patients show later onset of the disorder. Belongs to the band 7/mec-2 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q25.2
Cellular Component: intercellular junction; lipid raft; plasma membrane; protein complex
Molecular Function: protein binding
Biological Process: actin cytoskeleton reorganization
Disease: Nephrotic Syndrome, Type 2
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Product Notes
The NPHS2 nphs2 (Catalog #AAA140792) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: MKHHHHH HASVKV VQEYERV IIFRLGHLLP GRAKGPGLFF FLPCLDTYHK VDLRLQTLEI PFHEIVTKDM FIMEIDAICY YRMENASLLL SSLAHVSKAV QFLVQTTMKR LLAHRSLTEI LLERKSIAQD AKVALDSVTC IWGIKVERIE IKDVRLPAGL QHSLAVEAEA QRQAKVRMIA AEAEKAASES LRMAAEILSG TPAAVQLRYL HTLQSLSTEK PSTVVLPLPF DLLNCLSSPS NRTQGSLPFP SPSKPVEPLN PKKKDSPML. . It is sometimes possible for the material contained within the vial of "Nephrosis 2 Idiopathic Steroid-Resistant, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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