Human Niemann-Pick C1-like protein 1 ELISA Kit | NPC1L1 elisa kit

Human Niemann-Pick C1-like protein 1 (NPC1L1) ELISA Kit

Cat. Num. AAA284058

• Gene Names: NPC1L1; NPC11L1; SLC65A2
• Reactivity: Human
• Synonyms: Niemann-Pick C1-like protein 1; Human Niemann-Pick C1-like protein 1 (NPC1L1) ELISA Kit; NPC11L1; NPC1L1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human NPC1L1. No significant cross-reactivity or interference between Human NPC1L1 and analogues was observed.
• Sequence Length: 1332

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for NPC1L1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NPC1L1 in samples. An antibody specific for NPC1L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNPC1L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPC1L1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPC1L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 156231353
• NCBI GeneID: 29881
• NCBI Accession #: NP_001095118.1
• NCBI GenBank Nucleotide #: NM_001101648.1
• UniProt Accession #: Q9UHC9; Q17RV5; Q6R3Q4; Q9UHC8; A4D2J7; B7ZLE6; D3DVK9
• Molecular Weight: 140,420 Da
• NCBI Official Full Name: Niemann-Pick C1-like protein 1 isoform 2
• NCBI Official Synonym Full Names: NPC1 like intracellular cholesterol transporter 1
• NCBI Official Symbol: NPC1L1
• NCBI Official Synonym Symbols: NPC11L1; SLC65A2
• NCBI Protein Information: Niemann-Pick C1-like protein 1
• UniProt Protein Name: Niemann-Pick C1-like protein 1
• Protein Family: Niemann-Pick C1-like protein
• UniProt Gene Name: NPC1L1

NCBI Description

The protein encoded by this gene is a multi-pass membrane protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1) domain and a putative sterol-sensing domain (SSD) which includes a YQRL motif functioning as a plasma membrane to trans-Golgi network transport signal in other proteins. This protein takes up free cholesterol into cells through vesicular endocytosis and plays a critical role in the absorption of intestinal cholesterol. It also has the ability to transport alpha-tocopherol (vitamin E). The drug ezetimibe targets this protein and inhibits the absorption of intestinal cholesterol and alpha-tocopherol. In addition, this protein may play a critical role in regulating lipid metabolism. Polymorphic variations in this gene are associated with plasma total cholesterol and low-density lipoprotein cholesterol (LDL-C) levels and coronary heart disease (CHD) risk. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

NPC1L1: Play a major role in cholesterol homeostasis. Is critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte. Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorbtion. Lack of activity leads to multiple lipid transport defects. The protein may have a function in the transport of multiple lipids and their homeostasis, and may play a critical role in regulating lipid metabolism. Interacts with RAB11A, MYO5B and RAB11FIP2. Interaction with RAB11A, MYO5B and RAB11FIP2 is required for proper transport to the plasma membrane upon cholesterol depletion. Expression is decreased in Caco-2 cells upon PPARD activation. Widely expressed. Expressed in liver. Also expressed in small intestine, pancreas, kidney, lung, pancreas, spleen, heart, gall bladder, brain, testis, stomach and muscle. Belongs to the patched family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: apical plasma membrane; brush border membrane; cytoplasmic vesicle membrane; integral component of membrane; plasma membrane

Molecular Function: drug binding; myosin V binding; protein binding; Rab GTPase binding

Biological Process: cholesterol biosynthetic process; cholesterol transport; intestinal cholesterol absorption; lipoprotein metabolic process; response to drug